PLANT BIOLOGY
ANIMAL BIOLOGY
SUBSCRIPTION
E-SUBSCRIPTION
 
MAP
MAIN PAGE

 

 

 

 

Kuliagina Yu.I., Borkhunova E.N., Pozyabin S.V., Shilkin A.G., Voitekha M.A. Pathomorphological characterization of lens capsule tissues in animals after prevention of secondary cata racts: an experimental study. Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2023, Vol. 58, № 6, p. 1148-1157.
(how to cite this article)

doi: 10.15389/agrobiology.2023.6.1148eng

UDC: 619:617.7

 

PATHOMORPHOLOGICAL CHARACTERIZATION OF LENS CAPSULE TISSUES IN ANIMALS AFTER PREVENTION OF SECONDARY CATARACTS: AN EXPERIMENTAL STUDY

Yu.I. Kuliagina1, 2 ✉, E.N. Borkhunova1, S.V. Pozyabin1,
A.G. Shilkin2, M.A. Voitekha2

1Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, 23, ul. Akademika K.I. Skryabina, Moscow, 109472 Russia, e-mail nikole08@mail.ru (✉ corresponding author), borhunova@mail.ru, jippo77@mail.ru;
2Shilkin Center for Veterinary Ophthalmology, 13, Snezhnaya ul, Moscow, 129323 Russia, e-mail shilkin555@mail.ru, m.voy@bk.ru

ORCID:
Kuliagina Yu.I. orcid.org/0000-0001-5209-158X
Shilkin A.G. orcid.org/0000-0001-5423-0299
Borkhunova E.N. orcid.org/0000-0003-2355-8616
Voitekha M.A. orcid.org/0000-0002-6543-8770
Pozyabin S.V. orcid.org/0000-0003-1296-2840

Final revision received June 5, 2023
Accepted July 07, 2023

One of the current problems of veterinary science is the treatment and prevention of pathologies that reduce the working and productive qualities of especially valuable animals. In veterinary ophthalmology, it remains an important goal to find ways to prevent secondary cataract after ultrasound phacoemulsification. Different methods are used for this purpose, but the questions about pathomorphosis of secondary cataract and scientific basis of effective methods of its prevention remain open. In the present work morphologic changes in the lens capsule of rabbits 2 and 6 months after phacoemulsification under different methods of secondary cataract prophylaxis were identified for the first time. On the basis of clinical picture together with morphological changes the most effective method of prophylaxis - capsulorhexis of the posterior lens capsule - was established. The aim of the work was to study the features of morphological changes occurring in the lens capsule in rabbits after phacoemulsification combined with intraoperative techniques of secondary cataract prophylaxis. The study was carried out in 2020-2021 at the Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology named and at Dr. A.G. Shilkin Veterinary Ophthalmology Center on 18 crossbred rabbits (Oryctolagus cuniculus domesticus) of both sexes, aged 4 months, without ophthalmic pathologies. The animals were divided into three groups (6 animals in each group). Ultrasound phacoemulsification was performed in all groups according to the generally accepted technique. Animals of group 1 were implanted with intraocular lens (AquaFree Y-PL, hydrophobic, Overal Dia 13.0 mm, Rumex, USA), group 2 — with intracapsular ring (polymer ophthalmic intracapsular ring for lens bag spreading and lens capsule tensioning, KPV-“ETP-MG” ring No. 3, 13.0 mm, Federal State University Cross-Sectoral Research and Technology Centre, Acad. Fedorov Eye Microsurgery Rosmedtechnologiya-ETP-MG, Russia). In group 3 posterior capsulorhexis was performed. In the postoperative period, the rabbits received general (enrofloxacin, 5 mg/kg body weight, 2 times per day; ketoprofen, 3 mg/kg body weight, 1 time per day for 3 days) and topical treatment (atropine for 10 days; moxifloxacin, eye drops for 14 days; dexamethasone + neomycin + polymyxin B sulfate for 14 days; nepafenac for 28 days). The animals were under continuous monitoring, during which ophthalmologic examination and photographic fixation of the eye condition were performed. Signs of secondary cataract were identified by using slit-lamp biomicroscopy, the state of the ocular fundus was examined by ophthalmoscopy, and the transparency of the capsule was determined. The rabbits were subjected to drug euthanasia 2 and 6 months after the surgery, by 2 and 4 animals, respectively. Eyeballs were enucleated and placed in 10% neutralized formalin solution. For macroscopic evaluation of the state of the lens capsule, a slice was made in the area of the serrated edge of ora serrata with retinal capture. Most of the vitreous body was aspirated. After photography, a sagittal section of the eye was performed and subjected to histologic examination. It was found that within 2 months after performing the above techniques, proliferation of lens epithelium resumes in the capsule, lens fibers are formed (mainly in the area of the equator of the capsular bag), and adhesions with the iris may develop. Within 6 months, the changes progress, however, to a different degree depending on the technique used. It is shown that capsulorhexis of the posterior lens capsule is the most effective technique for secondary cataract prophylaxis due to a limited impact on tissues in general, absence of alien objects after the surgery and, therefore, the least proliferation of pathological lens tissues with preservation of optical transparency of the central region of the capsular bag.

Keywords: lens, cataract, posterior capsulorhexis, capsule opacification, secondary cataract, phacoemulsification, secondary cataract prevention.

 

REFERENCES

  1. Kulyagina Yu.I. Sravnitel’naya kharakteristika metodik intraoperatsionnoy profilaktiki vtorichnoy katarakty pri fakoemul’sifikatsii u sobak. Kandidatskaya dissertatsiya [Comparative characterization of methods for secondary cataract intraoperative prevention during phacoemulsification in dogs. PhD Thesis]. Moscow, 2022 (in Russ.).
  2. Gelatt K.N., Gelatt J.P., Plummer C. Veterinary Ophthalmic Surgery-E-Book. USA, 2011.
  3. Sigle K.J., Nasisse M.P. Long-term complications after phacoemulsification for cataract removal in dogs: 172 cases (1995-2002). Journal of the American Veterinary Medical Association, 2006, 228(1): 74-79 CrossRef
  4. Bras I.D., Colitz C.M.H., Saville W.J.A, Gemensky-Metzler A.J., Wilkie D.A. Posterior capsular opacification in diabetic and nondiabetic canine patients following cataract surgery. Veterinary Ophthalmology, 2006, 9(5): 317-327 CrossRef
  5. Apple D.J., Peng Q., Visessook N., Werner L., Pandey S.K., Escobar-Gomes M., Ram J., Auffarth G.U. Eradication of posterior capsule opacification: documentation of a marked decrease in Nd:YAG laser posterior capsulotomy rates noted in an analysis of 5416 pseudophakic human eyes obtained postmortem. Ophthalmology, 2020, 127(4): S29-S42 CrossRef
  6. Gabel V.P., Neubauer L., Zink H., Birngruber R. Ocular side effect following neodymium: YAG laser irradiation. International Ophthalmology Clinics, 1985, 25(3): 137-149 CrossRef
  7. Shihan M.H., Novo S.G., Duncan M.K. Cataract surgeon viewpoints on the need for novel preventative anti-inflammatory and anti-posterior capsular opacification therapies. Current Medical Research and Opinion, 2019, 35(11): 1971-1981 CrossRef
  8. Werner L., Apple D.J., Schmidbauer J.M. Ideal IOL (PMMA and Foldable). In: Phacoemulsification principles and techniques. L. Buratto, L. Werner, M. Zanini, D.J. Apple (eds.). Thorofare, NJ, 2003: 435-451.
  9. Yi N.-Y., Park S.-A., Jeong M.-B., Kim W.-T., Kim S.-E., Chae J.-M., Seo K.-M. Phacoemulsification and acryl foldable intraocular lens implantation in dogs: 32 cases. Journal of Veterinary Science, 2006, 7(3): 281-285 CrossRef
  10. Apple D.J. Peng Q., Visessook N., Werner L., Pandey S.K., Escobar-Gomez M., Ram J., Whiteside S.B., Schoderbeck R., Ready E.L., Guindi A. Surgical prevention of posterior capsule opacification: Part 1: progress in eliminating this complication of cataract surgery. Journal of Cataract & Refractive Surgery, 2000, 26(2): 180-187 CrossRef
  11. Gift B.W., English R.V., Nadelstein B., Weigt A.K., Gilger B.C. Comparison of capsular opacification and refractive status after placement of three different intraocular lens implants following phacoemulsification and aspiration of cataracts in dogs. Veterinary Ophthalmology, 2009, 12(1): 13-21 CrossRef
  12. Smolenskaya Yu.I., Pozyabin S.V. Veterinarnaya patologiya, 2020, 2(72): 48-54 (in Russ.).
  13. Ahmed I.I.K., Cionni R.J., Kranemann C., Crandall A.S. Optimal timing of capsular tension ring implantation: Miyake-Apple video analysis. Journal of Cataract & Refractive Surgery, 2005, 31(9): 1809-1813 CrossRef
  14. Martin C.L. Ophthalmic disease in veterinary medicine. CRC Press, London, 2009 CrossRef
  15. Morales A., da Costa P.F., da Conceição L.F., de Andrade A.L., Padua I.R.M., Marinho F.A., da Cunha Brito F.L., Laus J.L. Capsular tension ring alone and associated with acrylic foldable intraocular lens in posterior capsular opacification after phacoemulsification in dogs. Ciência Rural, Santa Maria, 45(3), 2015, 45(3): 540-545 CrossRef
  16. Bantsykina Yu.V., Malov I.V., Eroshevskaya E.B., Malov V.M. MPrakticheskaya meditsina, 2018, 3: 20-24 (in Russ.).
  17. Malyugin B.E. Vestnik oftmal’mologii, 2014, 6: 80-88 (in Russ.).
  18. Malov I.V., Eroshevskaya E.B., Malov V.M., Krikun T.I., Bantsykina Yu.V. Izvestiya Samarskogo nauchnogo tsentra Rossiyskoy akademii nauk, 2015, 5-3: 822-824 (in Russ.).
  19. Shilkin A.G., Pavlova T.N., Rotanov D.A., Novikova K.A. Veterinariya, zootekhniya i biotekhnologiya, 2015, 1: 46-50 (in Russ.).
  20. Galand A., van Cauwenberge F., Moosavi J. Posterior capsulorhexis in adult eyes with intact and clear capsules. Journal of Cataract & Refractive Surgery, 1996, 22(4): 458-461 CrossRef
  21. Zanini M., Buratto L., Savini G. Capsulorhexis. In: Phacoemulsification principles and techniques. L. Buratto, L. Werner, M. Zanini, D.J. Apple. Thorofare, NJ, 2003: 83-92.
  22. Colitz C.M.H., Malarkey D., Dykstra M.J., McGahan M.C., Davidson M.G. Histologic and immunohistochemical characterization of lens capsular plaques in dogs with cataracts. American Journal of Veterinary Research, 2000, 61(2): 139-143 CrossRef
  23. Gotoh N., Perdue N.R., Matsushima H., Sage E.H., Yan Q., Clark J.I. An in vitro model of posterior capsular opacity: SPARC and TGF-β2 minimize epithelial-to-mesenchymal transition in lens epithelium. Investigative Ophthalmology & Visual Science, 2007, 48(10): 4679-4687 CrossRef
  24. Bernays M.E., Peiffer R.L. Morphologic alterations in the anterior lens capsule of canine eyes with cataracts. American Journal of Veterinary Research, 2000, 61(12): 1517-1519 CrossRef
  25. Pozyabin S.V., Borkhunova E.N., Peryshkina L.S. Veterinariya, 2020, 6: 47-50 (in Russ.).
  26. Kozlov N., Pozyabin S., Bhattarai B., Lipták T., Fuchs J., Marešová S., Kuricová M. Surgical management of intervertebral disc extrusion in a rabbit. Journal of Small Animal Practice, 2022, 63(7): 570-570 CrossRef

 

back

 


CONTENTS

 

 

Full article PDF (Rus)

Full article PDF (Eng)