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Deniskova Т.Е., Shakhin A.V., Esmailizadeh A., Dotsev А.V., Zinovieva N.А. Analysis of polymorphism in the major genes for reproductive traits in sheep (Ovis spp.). Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2023, Vol. 58, № 6, p. 1046-1056.
(how to cite this article)

doi: 10.15389/agrobiology.2023.6.1046eng

UDC: 636.38:575.162

Acknowledgements:
During the research, the equipment of the Center for Biological Resources and Bioengineering of Agricultural Animals (Ernst Federal Research Center for Animal Husbandry) was used.
Sequences of the Iranian sheep complete genomes were provided by Professor A. Esmailizadeh, the Head of project No. 98028814 (from the Iranian side), implemented as part of a competition for the best basic research projects conducted by the Russian Foundation for Basic Research and the National Science Foundation of Iran.
Funded by RFBR and INSF (grant number No 98028814), within the project No. 20-516-56002

 

ANALYSIS OF POLYMORPHISM IN THE MAJOR GENES FOR REPRODUCTIVE TRAITS IN SHEEP (Ovis spp.)

Т.Е. Deniskova1, 2 ✉, A.V. Shakhin1, A. Esmailizadeh3,
А.V. Dotsev1, N.А. Zinovieva1

1Ernst Federal Research Center for Animal Husbandry, 60, pos. Dubrovitsy, Podolsk District, Moscow Province, 142132 Russia, e-mail horarka@yandex.ru (✉ corresponding author), alexshahin@mail.ru, asnd@mail.ru, n_zinovieva@mail.ru;
2Basic Department of Genetic Technologies in livestock Farming, Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, 23, ul. Akademika Skryabina, Moscow, 109472 Russia;
3Department of Animal Science, Shahid Bahonar University of Kerman, Iran, Kerman 76169-14111, e-mail aliesmaili@uk.ac.ir

ORCID:
Deniskova T.E. orcid.org/0000-0002-5809-1262
Dotsev A.V. orcid.org/0000-0003-3418-2511
Shakhin A.V. orcid.org/0000-0003-4959-878X
Zinovieva N.A. orcid.org/0000-0003-4017-6863
Esmailizadeh A. orcid.org/0000-0003-0986-6639

Final revision received October 16, 2023
Accepted November 06, 2023

The reproductive traits significantly affect the cost of production of sheep products. The BMP15, GDF9 and BMPR1B are the major genes for reproduction in sheep, mutations in which increase number of eggs per ovulation and litter size. The segregation of new mutations in an expanding breed diversity has been periodically reported. In this regard, the search for new SNPs in unexplored breeds is relevant to deepen knowledge about the genetic mechanisms underlying sheep prolificacy. In our work, for the first time, a comparative analysis of the complete nucleotide sequences of the GDF9, BMP15 and BMP15B genes in the Romanov sheep was carried out in comparison with other breeds of domestic sheep (Ovis aries L.) and wild relatives. The most significantly varied SNPs were identified based on comparing the Romanov breed with autochthonous breeds of domestic sheep from Russia and the Persian Highlands, as well as wild Ovis species. Polymorphism in the major genes for reproduction in sheep was studied for argali (O. ammon L.) and mouflon (O. orientalis L.) for the first time. SNPs fixed in argali and domestic sheep were identified. The studies were conducted at Ernst Federal Research Center for Animal Husbandry in 2022-2023. We analyzed whole genome sequences of domestic sheep, Romanov (n = 9), other Russian breeds (n = 7), Iranian breeds (n = 6) and wild Ovis species, the Asian mouflon (O. orientalis, n = 16) and argali (O. ammon, n = 4). Alignment to the reference genome was performed using bwa-mem2 and SAMtools. The sequences of the GDF9, BMP15, and BMP15B genes were extracted from the whole genomes, in which the most different SNPs were searched based on the calculation of FST values for each SNP for each pair of groups. Gene sequences comparison of the Romanov breed with other breeds showed that the greatest differences were identified in the BMPR1B gene (FST = 0.562-0.749) when compared to the BMP15 (FST = 0.051-0.374) and GDF9 genes(FST = 0.037-0.660). Comparative analysis of gene sequences in the Romanov sheep and argali showed the presence of fixed SNPs (FST = 1), while one such SNP was identified in the GDF9 gene. The highest FST values identified based on comparing Romanov breed sheep with mouflon were 0.702-0.780 (BMPR1B gene), 0.113-0.645 (BMP15 gene) and 0.338-0.512 (GDF9 gene). Thus, target SNPs were identified the effect of which on reproductive traits in the Romanov sheep should be studied in future work.

Keywords: SNP, candidate genes, Ovis aries, Ovis ammon, Ovis orientalis, domestic sheep, wild species, prolificacy.

 

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