Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2017, Vol. 52, № 6, p. 1206-1213
(how to cite this article)

doi: 10.15389/agrobiology.2017.6.1206eng

UDC 615.32: 57.085.23

Acknowledgements:
The experiments were carried out using equipment of the SIMBIOZ Center (BPPM RAS).
The study was implemented under the Basic Science Research Program (State Assignment № 0755-2015-0002) and supported financially in part by Russian Science Foundation (grant № 15-14-00002)

 

PREPARATION OF SELENIUM NANOPARTICLES BY USING
SILYMARIN AND STUDY OF THEIR CYTOTOXICITY TO TUMOR CELLS

S.A. Staroverov1, 2, L.A. Dykman1, 2, P.V. Mezhennyi3, A.S. Fomin1, 2,
S.V. Kozlov3, A.A. Volkov2, 3, A.O. Rybin2, 3, A.B. Golova2,
V.A. Khanadeev1, A.A. Kurilova3, S.Yu. Shchyogolev1, 4

1Institute of Biochemistry and Physiology of Plants and Microorganisms RAS, Federal Agency of Scientific Organizations, 13, prosp. Entuziastov, Saratov, 410049 Russia,
e-mail staroverovsergey@me.com, dykman_l@ibppm.ru, khanadeev_v@ibppm.ru, shegolev_s@ibppm.ru (corresponding author);
2Saratov Research Veterinary Institute RAS, Federal Agency of Scientific Organizations, 6, ul. 53 Strelkovoi divizii, Saratov, 410028 Russia, e-mail strazth87@bk.ru volkov-aleksei@yandex.ru, alinagolova@mail.ru;  
3N.I. Vavilov Saratov State Agrarian University, 1, Teatralnaya ploshchad, Saratov, 410012 Russia, e-mail v1rus-m@rambler.ru, kozlov12@inbox.ru, N89063171899@gmail.com, goffa2009@yandex.ru;
4N.G. Chernyshevsky National Research Saratov State University, 83, ul. Astrakhanskaya, Saratov, 410012 Russia


ORCID:
Staroverov S.A. orcid.org/0000-0002-4752-9855
Dykman L.A. orcid.org/0000-0003-2440-6761

Received December 12, 2016

 

The past few years have seen substantial progress in veterinary oncology: new methods have been developed for the diagnosis and treatment of oncological diseases in animals, and the range of possible therapeutic interventions has been broadened. Of interest, in particular, are the prospects for the creation of veterinary pharmaceuticals with the use of various nanoparticles, including colloidal selenium, on the surface of which are immobilized biologically active substances that have antitumor action. Selenium nanoparticles are cytotoxic to tumor cells and have also been considered as effective carriers for the in vivo targeted delivery of drugs, genetic materials, proteins, and so on. The well-tunable polyvalent structures of the selenium nanoparticle surface provide a convenient platform for integrating several therapeutic agents or biomacromolecules with covalent or noncovalent surface conjugation. We synthesized selenium nanoparticles in complex with silymarin, a flavonoid hepatoprotector extracted from the fruit of milk thistle [Silybum marianum (L.) Gaertn.], and we evaluated the cytotoxicity of the resultant preparation to normal and tumorous cells. By using electron microscopy and dynamic light scattering, it was found that the developed procedure ensured the preparation of stable suspensions of silymarin-conjugated selenium nanoparticles with sizes ranging from 20 to 40 nm. The obtained conjugate was shown to be markedly cytotoxic to the Hep-2 tumor cell line, suppressing cell respiration approximately 6.5-fold as compared to the control, whereas the respiration of SPEV-2 normal cells was inhibited approximately 2.3-fold. Initial colloidal selenium had much weaker effects on both cell types, and pure silymarin had no statistically significant influence on SPEV-2 cells (in contrast to Hep-2 cells). The results of this study could be used in developing next-generation anticancer agents and are of interest in the implementation of green chemistry-based approaches.

Keywords: Silybum marianum, flavonolignans, silymarin, selenium nanoparticles, conjugation, cytotoxic effects.

 

Full article (Rus)

Full article (Eng)

 

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