УДК 638.1:636.082:575.174.015.3
MICROSATELLITES AS A TOOL FOR EVALUATION OF ALLELE POOL DYNAMICS WHEN CREATION OF PRIOKSKY TYPE OF MIDDLE RUSSIAN HONEY BEE
N.A. Zinovieva1, N.I. Krivtsov2, M.S. Fornara1, 3, E.A. Gladyr’1, 3, A.V. Borodachev2, S.A. Berezin2, V.I. Lebedev2
The informative capacity of the test system for the analysis of seven microsatellites (A024, A88, A113, AP043, HB-C16-05, HB-THE-03, HB-C16-01) for the allele pool characteristics of Middle Russian (n = 65), Gray Mountain Caucasian (n = 70) and Prioksky type of Middle Russian (n = 88) honey bee breeds was studied. The average number of alleles, the number of informative alleles and the number of effective alleles per loci were 7.48±1.02, 3.62±0.71 and 3.38±0.56, respectively. The increase of allele pool genetic diversity in Prioksky type comparing to Middle Russian and Grey Mountain Caucasian breeds used in type breeding was observed: 9.57±1.88 allele per loci as against 6.86±1.55 and 6.00±1.84, respectively. The allele introduction of initial breeds was evaluated. It was shown that 7.9 per cent of common microsatellite diversity was due to intra population differences.
Keywords: genetic markers, microsatellites, heterogeneity, honey bee breeds.
1ГНУ Всероссийский НИИ животноводства |
Поступила в редакцию |