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Diakite S., Polyakov A.V., Stakheev A.A., Alekseeva T.V., Zavriev S.K., Said R.R. Species composition of fungi of the genus Fusarium Link on garlic plants in Moscow region. Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2022, Vol. 57, № 1, p. 151-157.
(how to cite this article)

doi: 10.15389/agrobiology.2022.1.151eng

UDC: 635.262:632.4.01/08

Acknowledgements:
Supported financially in part from the Russian Foundation for Basic Research (grant No. 19-04-00642)

 

SPECIES COMPOSITION OF FUNGI OF THE GENUS Fusarium Link ON GARLIC PLANTS IN MOSCOW REGION

S. Diakite1, A.V. Polyakov2, 3, A.A. Stakheev4 ✉, T.V. Alekseeva2, 3,
S.K. Zavriev4, R.R. Said5

1People’s Friendship University of Russia (RUDN University), 6, ul. Miklukho-Maklaya, Moscow, 117198 Russia, e-mail diakitesimbo8@gmail.com;
2Moscow Region State University, 24, ul. Very Voloshinoi, Mytishchi, Moscow Province, 141014 Russia, e-mail vita100plus@yandex.ru,
kaf-bosh@mgou.ru;
3All-Russian Research Institute of Vegetable Growing — Branch of the Federal Scientific Vegetable Center, str. 500, Vereya, Ramenskii District, Moscow Province, 140153 Russia, e-mail vita100plus@yandex.ru,
kaf-bosh@mgou.ru;
4Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 16/10, ul. Miklukho-Maklaya, Moscow, 117997 Russia, e-mail Stakheev.aa@gmail.com (✉ corresponding author), szavriev@ibch.ru;
5Shaikh Zayed University, Faculty of Education, Department of Biology, Khost 2501, Afghanistan, e-mail rahmatullah.abid@szu.edu.af

ORCID:
Diakite S. orcid.org/0000-0003-1462-1329
Alekseeva T.V. orcid.org/0000-0002-9252-3153
Polyakov A.V. orcid.org/0000-0002-5413-0770
Zavriev S.K. orcid.org/0000-0002-6741-8175
Stakheev A.A. orcid.org/0000-0002-0732-5321
Said R.R. orcid.org/0000-0001-7620-899X

December 7, 2021

 

Garlic (Allium sativum L.) cultivation is restrained by the lack of seed propagation and high sensitivity to pathogenic fungi, bacteria, viruses and nematodes. Currently, Fusarium dry rot caused by soil fungi of the genus Fusarium is the most harmful disease of the crop. For the first time, in a multidisciplinary study, we have quantified the ratio of the main Fusarium dry rot pathogens affecting garlic in the Moscow region. Data shows the percentage of occurrence of key pathogens, high harmfulness and a dominant position of dry rot of the species F. proliferatum in the complex of pathogens. The work aimed to investigate the species composition of plant pathogenic fungi of the genus Fusarium on a garlic (Allium sativum L.) crop in the Moscow region. Diseased bulbs (n = 1108) of winter garlic cv. Gladiator were collected during 2019 to 2021 (All-Russian Research Institute of Vegetable Growing) and divided into sample sets based on the same symptoms of bulbs and cloves damage. Two hundred garlic cloves from each sample were surface sterilized by common methods and used to isolate pathogens. Scrapings from damaged clove tissues were placed on Czapek’s medium in sterile Petri dishes and incubated for 12 days at 25 °C. The colonies were subcultured until monospore cultures were obtained. The isolates were identified using taxonomic keys. The frequency of occurrence of pathogens was calculated. Analysis of the marker sequences of the TEF1a gene (translation elongation factor 1 alpha) (~ 550 bp) and MCM7 (gene encoding minichromosome maintenance protein 7) (~ 650 bp) was performed to confirm the taxonomic identification of the isolates. Molecular identification of the isolates was performed by quantitative PCR using species-specific primersfor F. proliferatum. NCBI’s BLAST algorithm (https://blast.ncbi.nlm.nih.gov/Blast.cgi) was used to analyse the nucleotide sequences of the TEF1a and MCM7 genes. Seven genera of fungi, the Fusarium, Penicillium, Botrytis, Embellisia, Aspergillus, Alternaria, and Sclerotium were isolated from the garlic bulbs with varying degrees of occurrence. Among these pathogens, the genus Fusarium prevailed with a frequency of more than 44 %. Of six identified species, the F. proliferatum, F. oxysporum, F. poae, F. verticilloides, F. culmorum and F. acuminatum, the F. proliferatum predominated at a frequency of 61.4-75.6 %. Sequencing of DNA markers confirmed identification of the F. proliferatum isolates. The similarity to the sequences deposited in the NCBI database was 99-100 % for TEF1a gene (reference sequence numbers MN158137, KP267240, MN012923, KT224299) and 98-99 % for MCM7 gene  (reference sequence numbers XM031230017, XM0311M36726). qPCR analysis using specific primers also confirmed these results. Our findings add on the available data on the prevalence and dynamics of changes in the species composition of fungi of the genus Fusarium in the Moscow region. The data are also practically important for the development of more effective methods to prevent and control Fusarium diseases of garlic.

Keywords: garlic, Fusarium genus,Fusarium dry rot, frequency of occurrence, DNA markers, polymerase chain reaction.  

 

REFERENCES

  1. Galvez Patón L. Etiología, epidemiología y estrategias de control de la podredumbre del diente de ajo (Allium sativum L.). Ingeniera Agrónoma, Madrid, 2017 CrossRef
  2. Alekseeva T.V., Diakite S., Polyakov A.V. Materialy Mezhdunarodnoi nauchno-prakticheskoi konferentsii «Innovatsii v sel'skom khozyaistve i ekologii» [Proc. Int. Conf. «Innovations in agriculture and ecology»]. Ryazan', 2020: 8-11 (in Russ.).
  3. Shestakova K.S. Selektsionno-immunologicheskaya kharakteristika ustoichivosti chesnoka ozimogo (Allium sativum L.) k fuzarioznoi gnili. Avtoreferat kandidatskoi dissertatsii [Selection and immunological characteristics of the resistance of winter garlic (Allium sativum L.) to fusarium rot. PhD Thesis]. Moscow, 2009 (in Russ.).
  4. Seredin T.M., Gerasimova L.I., Kozar' E.G., Engalycheva I.A., Baranova E.V. Ovoshchi Rossii, 2018, 6: 84-90 CrossRef (in Russ.).
  5. Dugan F.M., Lupien S.L., Hellier B.C. Infection by Fusarium proliferatum in aerial garlic bulbils is strongly reduced compared to rates in seed cloves when both originate from infected bulbs. Crop Protection, 2019, 116: 43-48 CrossRef
  6. Filyushin M.A., Anisimova O.K., Kochieva E.Z., Shchennikova A.V. Genome-wide identification and expression of chitinase class I genes in garlic (Allium sativum L.) cultivars resistant and susceptible to Fusarium proliferatum. Plants, 2021, 10(4): 720 CrossRef
  7. Aoki T., O’Donnell K., Geiser D.M. Systematics of key phytopathogenic Fusarium species: status and future challenges. Journal of General Plant Pathology,2014, 80(3): 189-201 CrossRef
  8. Munkvold G.P. Fusarium species and their associated mycotoxins. In: Mycotoxigenic fungi. Methods and protocols, vol. 1542. A. Moretti, A. Susca (eds.). Humana Press, NY, 2017: 51-106 CrossRef
  9. Cobo-Díaz J.F., Baroncelli R., Le Floch G., Picot A. A novel metabarcoding approach to investigate Fusarium species composition in soil and plant samples. FEMS Microbiology Ecology, 2019, 95(7): fiz084 CrossRef
  10. Palmero D., de Cara M., Nosir W., Galez Paton L., Cruz A., Woodward S., Gonzalez-Jaen M.T., Tello J.C. Fusarium proliferatum isolated from garlic in Spain: Identification, toxigenic potential and pathogenicity on related Allium species. Phytopathologia Mediterranea, 2012, 51(1): 207-218.
  11. O’Donnell K., Rooney A.P., Proctor R.H., Brown D.W., McCormick S.P., Ward T.J., Frandsen R.Jn., Lysoe E., Rehner S., Aoki T., Robert V., Crous P.W., Groenewald J.Z., Kang S., Geiser D.M. Phylogenetic analyses of RPB1 and RPB2 support a middle Cretaceous origin for a clade comprising all agriculturally and medically important fusaria. Fungal Genetics and Biology, 2013, 52: 20-31 CrossRef
  12. Gosudarstvennyi reestr selektsionnykh dostizhenii, dopushchennykh k ispol'zovaniyu. Tom 1. Sorta rastenii [The State Register of breeding accomplishments approved for use. Volume 1. Plant varieties]. Moscow, 2021: 375-377 (in Russ.).
  13. Le D., Audenaert K., HaesaertG. Fusarium basal rot: profile of an increasingly important disease in Allium spp. Tropical Plant Pathology, 2021, 46: 241-253 CrossRef
  14. Mondani L., Chiusa G., Battilani P. Chemical and biological control of Fusarium species involved in garlic dry rot at early crop stages. European Journal of Plant Pathology, 2021, 160: 575-587 CrossRef
  15. Bjelić D., Ignjatov M., Marinković J., Milošević D., Nikolić Z., Gvozdanović-Varga J., Karaman M. Bacillus isolates as potential biocontrol agents of Fusarium clove rot of garlis. Zemdirbyste-Agriculture, 2018, 105(4): 369-376 CrossRef
  16. Seredin T.M., Kozar' E.G., Gerasimova L.I., Engalycheva I.A. Ovoshchi Rossii, 2018, 6: 84-90 CrossRef
  17. Litvinov S.S. Metodika polevogo opyta v ovoshchevodstve [Methodology of field experience in vegetable growing]. Moscow, 2011 (in Russ.).
  18. Diakite S., Polyakov A.V., Alekseeva T.V., Azopkova M.A., Murav'eva I.V. MaterialyVserossiiskoinauchno-prakticheskoikonferentsii s mezhdunarodnym uchastiem, posvyashchennoi professoru Yu.D. Zhilovu «Ekologiya i zdorov'e cheloveka» [Proc. Russian Conf. with international participation, dedicated to professor Yu.D. Zhilov «Ecology and human health»]. Moscow, 2020: 93-97 (in Russ.).
  19. Leslie J.F., Summerell B.A. The Fusarium laboratory manual. Blackwell Publishing, 2006.
  20. Stakheev A.A., Samokhvalova L.V., Mikityuk O.D., Zavriev S.K. ActaNaturae, 2018, 10(2): 79-92 CrossRef (in Russ.).
  21. Stakheev A.A., Khairulina D.R., Zavriev S.K. Four-locus phylogeny of Fusarium avenaceum and related species and their species-specific identification based on partial phosphate permease gene sequences. International Journal of Food Microbiology, 2016, 225: 27-37 CrossRef
  22. Mondani L., Chiusa G., Battilani P. Fungi associated with garlic during the cropping season, with focus on Fusarium proliferatum and F. oxysporum. Plant Health Progress, 2021, 22(1): 37-46 CrossRef
  23. Dugan F.M., Hellier B.C., Lupien S.L. First report of Fusarium proliferatum causing rot of garlic bulbs in North America. Plant Pathology, 2003, 52(3): 426 CrossRef
  24. Filyushin M.A., Danilova O.A., Seredin T.M. Ovoshchi Rossii, 2021, 3: 105-109 CrossRef (in Russ.).
  25. Seefelder W., Gossmann M., Humpf H. Analysis of fumonisin B1 in Fusarium proliferatum-infected asparagus spears and garlic bulbs from Germany by liquid chromatography-electrospray ionization mass spectrometry. Journal of Agricultural and Food Chemistry, 2002, 50(10): 2778-2781 CrossRef
  26. Stankovic S., Levic J., Petrovic T., Logrieco A., Moretti A. Pathogenicity and mycotoxin production by Fusarium proliferatum isolated from onion and garlic in Serbia. European Journal of Plant Pathology, 2007, 118(2): 165-172 CrossRef
  27. Tonti S., Dal Prà M., Nipoti P., Prodi A., Alberti, I. First report of Fusarium proliferatum causing rot of stored garlic bulbs (Allium sativum L.) in Italy. Journal of Phytopathology, 2012, 160: 761-763 CrossRef
  28. Gálvez L., Palmero D. Incidence and etiology of postharvest fungal diseases associated with bulb rot in garlic (Alllium sativum) in Spain. Foods, 2021, 10(5): 1063 CrossRef
  29. Sankar N.R., Babu G.P. First report of Fusarium proliferatum causing rot of garlic bulbs (Allium sativum) in India. Plant Disease, 2012, 96(2): 290 CrossRef
  30. Moharam M.H.A., Farrag E.S.H., Mohamed M.D.A. Patogenetic fungi in garlic seed cloves and first report of Fusarium proliferatum causing cloves rot of stored bulbs in upper Egypt. Archives of Phytopathology and Plant Protection, 2013, 46(17): 2096-2103 CrossRef
  31. Leyronas C., Chrétien P.L., Troulet C., Duffaud M., Villeneuve F., Morris C.E., Hunyadi H. First report of Fusarium proliferatum causing garlic clove rot in France. Plant Disease, 2018, 102(12): 2658 CrossRef

 

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