doi: 10.15389/agrobiology.2019.1.110eng

UDC 632.937:632.937.33:575.21

The studies were performed according to the State Task No. 075-00376-19-00 of the Ministry of Science and Higher Education of the Russian Federation within the framework of scientific research work on the subject No 0686-2019-0009



V.Ya. Ismailov1, I.S. Agas’eva1, V.I. Kil2, E.V. Fedorenko1,
M.V. Nefedova1,E.N. Besedina1

1All-Russian Research Institute of Biological Plant Protection, PO box 39, Krasnodar, 350039 Russia, e-mail,,,,, (✉ corresponding author);
2Kuban State University, 149, ul. Stavropolskaya, Krasnodar, 350040 Russia, e-mail (✉ corresponding author)

Ismailov V.Ya.
Fedorenko E.V.
Agas’eva I.S.
Nefedova M.V.
Kil V.I.
Besedina E.N.

Received March 20, 2018


Perillus bioculatus Fabr. (1775) is a predatory bug in the family Pentatomidae, a prospective North American entomophage of the Colorado potato beetle. Earlier, Colorado potato beetle eggs were deemed the most preferable for the predator feeding and the main obstacle for perillus acclimatization on new territories. However, in monitoring perillus populations that have acclimatized in several areas of Krasnodar region for the last decade, we revealed the predators’ feeding on ragweed leaf beetle (Zygogramma suturalis Fabr.) larvae and ragweed cutworm Tarachidia candefacta Hübn. (NoctuidaeLepidoptera) caterpillars and also on all growth stages of Colorado potato beetle and other insects. In Krasnodar Krai perillus can give annually three generations and is present in three phenoforms, with red-black, orange-black and white-black scutellum. The abundance of P. bioculatus is influenced by feeding conditions and by other entomophages, e.g. egg-eaters of genus Trissolcus (Hymenoptera: Scelionidae) and tachina flies (Diptera: Tachinidae). Analysis of scutellum color inheritance in seven different crosses, including reciprocal ones, between red, orange (or yellow) and white parents showed that the F1 and F2 progeny from all combinations, including white individuals crossed with each other, have only red and orange shield (1:1). PCR analysis of P. bioculatus bugs in F2 from crossing insects of different phenoforms revealed that the bugs with the same phenoform in F2 are genetically different from each other for the frequency of certain RAPD markers. These data indicate non-Mendelian inheritance of scutellum coloration trait in perillus that additionally attract attention to the point. RAPD (random amplification of polymorphic DNA)- and ISSR(inter simple sequence repeat)-PCR analysis detected intra- and inter-population variation of the predatory bugs molecular genetic structure from different zones of the Krasnodar Krai. PCR analysis with almost all used specific primers revealed statistically significant differences (p = 0.05) in molecular genetic structure and DNA polymorphism for the populations studied. Geographic populations from the village of Staro-Nizhesteblievskaya and the city of Krasnodar (Krasnodar Krai) were genetically close while there genetic identities with the Moldavian village (Crimean region, Krasnodar Krai) population were low. This comports with their geographical location and indicates geographic variability of P. bioculatus populations’ genetic structure. The researches executed by us confirm plasticity of this species and prospects for its further acclimatization within Colorado potato beetle area.

Keywords: predatory bugs, Perillus bioculatus Fabr., adventive species, acclimatization, PCR analysis, molecular genetic similarity, Colorado beetle, entomophages, biocontrol.




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