УДК 635.21:581.143.6:57.086.83
FORMATION AND MORPHOMETRIC INDICES OF POTATO MICROTUBERS in vitro AT VARIOUS FORMULA OF SUGARS IN MEDIUM
A.N. Deryabin, N.O. Yur’eva
On aseptic culture of potato stolons of the Zhukovskii early and Lugovskoi varieties, isolated in roller in absolute darkness, the authors studied the effect of glucose, sucrose and fructose, supplemented to nutrient medium of Murashige-Skoog (MS-medium), on formation of stolons and tubers. The appearance of only axillary microtubers (40 % — in leaf axil of stem explants and 60 % — in leaf axil of first internode of stolon) the authors observed at the induction of tuber formation by MS-medium with 8 % of sucrose. For the raising of efficiency of tuber formation and the improvement of morphometric indices of microtubers the authors suggest MS-medium with 8 % sucrose, which induces the tuber formation at 7 day of cultivation, replace entirely on analogous in 15-20 days after induction.
Keywords: Solanum tuberosum L., microtubers, stolon development, tuberization, sucrose, glucose, fructose.
K.A. Timiryazev Institute of Plant Physiology, RAS, Moscow 127276, Russia e-mail: anderyabin@mail.ru |
Received January 13, 2009 |
