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Dubrovina I.A., Moshkina E.V. Impact of biochar on functional activity dynamics of microbial communities in boreal soils. Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2024, Vol. 59, № 5, p. 943-954.
(how to cite this article)

doi: 10.15389/agrobiology.2024.5.943eng

UDC: 631.46

Acknowledgements:
Performed using analytical equipment of Core Facility of the Karelian Research Center RAS within the framework of state task No. FMEN-2022-0012.

 

IMPACT OF BIOCHAR ON FUNCTIONAL ACTIVITY DYNAMICS OF MICROBIAL COMMUNITIES IN BOREAL SOILS

I.A. Dubrovina1 ✉, E.V. Moshkina2

1Institute of Biology, Karelian Research Centre RAS, 11, Pushkinskaya, Petrozavodsk, 185910 Russia, e-mail vorgo@mail.ru (✉ corresponding author);
2Forest Research Institute, Karelian Research Centre RAS, 11, Pushkinskaya, Petrozavodsk, 185910 Russia, e-mail lena_moshkina@mail.ru

ORCID:
Dubrovina I.A. orcid.org/0000-0001-7229-6611
Moshkina E.V. orcid.org/0000-0003-3752-4126

Final revision received November 15, 2023
Accepted January 11, 2024

Biochar is the product of the pyrolysis of various organic materials with high carbon contents. Owing to its aromatic structure, high porosity, and cation exchange capacity, biochar has a significant impact on the physical, chemical, and biological properties of soils. The use of biochar in agriculture provides an opportunity for safe waste disposal, soil quality, and carbon sequestration. One of the few studied consequences of biochar application is the change in the structure, abundance, and ecophysiological status of soil microorganisms. In this study, we established for the first time that biochar has a multidirectional effect on the functioning of boreal soil microbial communities. The purpose of this study was to study the effects of wood biochar on basal respiration, microbial biomass carbon, and the microbiological quotients of boreal soils of various textures. The studies were conducted in the middle taiga subzone of Karelia. The sites were laid out in 2018 and 2019 on arable land plots with soils of different textures and normal moisture. The coarse soil texture was represented by the loamy sand Umbric Podzol on lake sands (UP) (Laboratory of Agrotechnology Vilga, Karelian Research Center RAS, Vidany settlement, Pryazhinsky District, Karelia, 61°52'22.8'' N, 34°0'36'' E). The fine soil texture was represented by the clay loam Umbric Retisol on loamy moraine (UR) (Agrobiological Station, Karelian Research Center RAS, Petrozavodsk, 61°45'3.6'' N, 34°21'12.4'' E). Plots of biochar and control sites with areas of 5 m2 were laid out randomly in four replicates. Birch biochar (GOST 7657-84), grade A (Russia) at a dose of 1.5 kg/m2 (15 t/ha) with a fraction size £ 1 cm was used in the study. Biochar was applied once and dug manually to a depth of 20-25 cm. No additional fertilizers were used. Potatoes were grown in the first year, and green manure was grown in the second year. Soil samples were collected monthly during the vegetation periods of 2018-2019 for plots with UP and 2019-2020 for plots with UR. Soil was collected from a depth of 5-20 cm in the arable horizon using the “envelope” method from each plot, and the mixed samples were analyzed in four replicates. The content of organic carbon (Corg.) by high-temperature catalytic combustion in a TOC-L CPN (“Shimadzu”, Japan) analyzer was determined. The rate of basal (microbial) respiration (BR) was measured in native soil incubated for 24 h at 22 °C. The content of microbial biomass carbon (Cmic.) was determined using the substrate-induced respiration method and was estimated using the rate of initial maximum respiration of microorganisms after enrichment of the soil with glucose (incubation for 1.5-2 h at 22 °C). Changes in CO2 concentration was recorded with a NDIR gas analyzer (SenseAir, Sweden). The parameters of the ecophysiological status of the microbial community were determined: the share of microbial biomass carbon in soil organic carbon Cmic./Corg., the metabolic quotient qCO2, and microbial respiration quotient QR. A study of the functional activity of microbial communities revealed the multifactorial and nonlinear effects of environmental conditions on the dynamics of the studied indicators at two-year monitoring sites in soils of different textures. Basal respiration was less dependent on weather conditions than microbial biomass carbon and related quotients, the magnitude of which was also influenced by organic residue supply. UP had lower microbial biomass, a less mature microbial community, lower stability, and lower organic matter quality than UR. The application of biochar has different effects on these soils. Despite the stable and reliable increase (р £ 0,05) in the total organic carbon of both soils (up to 20 %), when BC was added to UR, the BR, Cmic., and microbial quotients tended to significantly decrease (р £ 0,05), reflecting the lack of substrates available for microorganisms. At the same time, the qCO2 and QR indicators decreased slightly but nonsignificantly. There was an increase in BR (not statistically significant) and Cmic. (significant at р £ 0.05 for some dates) in UP, apparently reflecting a surge in K-strategist activity during the mineralization of hard-to-reach biochar compounds. The addition of biochar did not affect the microbial quotient of UP. At the same time, a decrease in the metabolic and microbial respiration quotients indicated that more favorable environmental conditions were being developed for microorganisms. Overall, biochar supplementation increased the adaptive potential of UP microbial communities.

Keywords: biochar, basal respiration, microbial biomass carbon, soil organic carbon, microbial quotient, metabolic quotient, microbial respiration quotient.

 

REFERENCES

  1. Gul S., Whalen J.K., Thomas B.W., Sachdeva V., Deng H. Physico-chemical properties and microbial responses in biochar-amended soils: mechanisms and future directions. Agriculture, Ecosystems and Environment, 2015, 206: 46-59 CrossRef
  2. Kuppusamy S., Thavamani P., Megharaj M., Venkateswarlu K., Naidu R. Agronomic and remedial benefits and risks of applying biochar to soil: current knowledge and future research directions. Environment International, 2016, 87: 1-12 CrossRef
  3. Hawthorne I., Johnson M.S., Jassal R.S., Black T.A., Grant N.J., Smukler S.M. Application of biochar and nitrogen influences fluxes of CO2, CH4 and N2O in a forest soil. Journal of Environmental Management, 2017, 192: 203-214 CrossRef
  4. Liu X., Zheng J., Zhang D., Cheng K., Zhou H., Zhang A., Li L., Joseph S., Smith P., Crowley D., Kuzyakov Y., Pan G. Biochar has no effect on soil respiration across Chinese agricultural soils. Science of the Total Environment, 2016, 554-555: 259-265 CrossRef
  5. Šlapakova B., Jeřabkova J., Vořišek K., Tejnecky V., Drabek O. The biochar effect on soil respiration and nitrification. Plant, Soil and Environment, 2018, 64(3): 114-119 CrossRef
  6. Walkiewicz A., Kalinichenko K., Kubaczyński A., Brzezińska M., Bieganowski A. Usage of biochar for mitigation of CO2 emission and enhancement of CH4 consumption in forest and orchard Haplic Luvisol (Siltic) soils. Applied Soil Ecology, 2020, 156: 103711 CrossRef
  7. Buchkina N.P., Balashov E.V., Šimanský V., Igaz D., Horák J. Changes in biological and physical parameters of soils with different texture after biochar application. Sel'skokhozyaistvennaya biologiya [Agricultural Biology], 2017, 52(3): 471-477 CrossRef
  8. Dubrovina I.A. Pochvovedenie, 2021, 12: 1523-1534 CrossRef (in Russ.).
  9. Khadem A., Raiesi F. Responses of microbial performance and community to corn biochar in calcareous sandy and clayey soils. Applied Soil Ecology, 2017, 114: 16-27 CrossRef
  10. Wu D., Senbayram M., Zang H., Ugurlar F., Aydemir S., Brüggemann N., Kuzyakov Y., Bol R., Blagodatskaya E. Effect of biochar origin and soil pH on greenhouse gas emissions from sandy and clay soils. Applied Soil Ecology, 2018, 129: 121-127 CrossRef
  11. Zhou H., Zhang D., Wang P., Liu X., Cheng K., Li L., Zheng J., Zhang X., Zheng J., Crowley D., Van Zwieten L., Pan G. Changes in microbial biomass and the metabolic quotient with biochar addition to agricultural soils: a meta-analysis. Agriculture, Ecosystems and Environment, 2017, 239: 80-89 CrossRef
  12. Wang Z., Zhao M., Yan Z., Yang Y., Niklas K.J., Huang H., Mipam T.D., He X., Hu H., Wright S.J. Global patterns and predictors of soil microbial biomass carbon, nitrogen, and phosphorus in terrestrial ecosystems. Catena, 2022, 211: 106037 CrossRef
  13. Rizhiya E.Ya., Boitsova L.V., Vertebniy V.E., Horak J., Moskvin M.A., Dubovitskaya V.I., Khomyakov Yu.V. Effect of biochar application on variability of the polyphenoloxidase and peroxidase activity of sod-podzolic soil under low andhighfertility. Sel'skokhozyaistvennaya biologiya [Agricultural Biology], 2022, 57(3): 476-485 CrossRef
  14. Oladele S.O., Adetunji A.T. Agro-residue biochar and N fertilizer addition mitigates CO2-C emission and stabilized soil organic carbon pools in a rain-fed agricultural cropland. International Soil and Water Conservation Research, 2021, 9(1): 76-86 CrossRef
  15. Sorrenti G., Buriani G., Gaggìa F., Baffoni L., Spinelli F., Gioia D., Toselli M. Soil CO2 emission partitioning, bacterial community profile and gene expression of Nitrosomonas spp. and Nitrobacter spp. of a sandy soil amended with biochar and compost. Applied Soil Ecology, 2017, 112: 79-89 CrossRef
  16. Atlas Karel’skoy ASSR /Pod redaktsiey A.G. Durova [Atlas of the Karelian ASSR. A.G. Durov (ed.)]. Moscow, 1989 (in Russ.).
  17. Anderson J.P.E., Domsch K.H. A physiological method for the quantitative measurement of microbial biomass in soils. Soil Biology & Biochemistry, 1978, 10(3): 215-221 CrossRef
  18. Karel’skiy tsentr po gidrometeorologii i monitoringu okruzhayushchey sredy. Informatsionno-analiticheskiy obzor gidrometeorologicheskikh usloviy na territorii Respubliki Kareliya [Information and analytical review of hydrometeorological conditions in the Republic of Karelia]. Available: https://www.kareliameteo.ru/press-center.html. Accessed: 02/02/2021 (in Russ.).
  19. Xu X., Thornton P.E., Post W.M. A global analysis of soil microbial biomass carbon, nitrogen and phosphorus in terrestrial ecosystems. Global Ecology and Biogeography, 2013, 22(6): 737-749 CrossRef
  20. Zhu L.-X., Xiao Q., Cheng H.-Y., Shi B.-J., Shen Y.-F., Li S.-Q. Seasonal dynamics of soil microbial activity after biochar addition in a dryland maize field in North-Western China. Ecological Engineering, 2017, 104(Part A): 141-149 CrossRef
  21. Wang Y., Liu X., Chen F. Huang R., Deng X., Jiang Y. Seasonal dynamics of soil microbial biomass C and N of Keteleeria fortunei var. cyclolepis forests with different ages. Journal of Forestry Research, 2020, 31: 2377-2384 CrossRef
  22. Dequiedt S., Saby N.P.A., Lelievre M., Jolivet C., Thioulouse J., Toutain B., Arrouays D., Bispo A., Lemanceau P., Ranjard L. Biogeographical patterns of soil molecular microbial biomass as influenced by soil characteristics and management. Global Ecology and Biogeography, 2011, 20(4): 641-652 CrossRef
  23. Pomazkina L.V., Semenova Yu.V. Pochvovedenie, 2018, 5: 617-629 CrossRef (in Russ.).
  24. Liu S., Zhang Y., Zong Y., Hu Z., Wu S., Zhou J., Jin Y., Zou J. Response of soil carbon dioxide fluxes, soil organic carbon and microbial biomass carbon to biochar amendment: a meta-analysis. GCB Bioenergy: Bioproducts for a Sustainable Bioeconomy, 2016, 8(2): 392-406 CrossRef
  25. Palansooriya K.N., Wong J.T.F., Hashimoto Y., Huang L., Rinklebe J., Chang S.X., Bolan N., Wang H., Ok Y.S. Response of microbial communities to biochar-amended soils: a critical review. Biochar, 2019, 1: 3-22 CrossRef
  26. Deshoux M., Sadet-Bourgeteau S., Gentil S., Prévost-Bouré N.C. Effects of biochar on soil microbial communities: a meta-analysis. Science of The Total Environment, 2023, 902: 166079 CrossRef
  27. Mukhina I.M., Durova A.S. Agrofizika, 2017, 1: 26-35 (in Russ.).">CrossRef
  28. Lavoie M., Mack M., Schuur E. Effects of elevated nitrogen and temperature on carbon and nitrogen dynamics in Alaskan arctic and boreal soils. Journal of Geophysical Research, 2011, 116(G3): G03013 CrossRef
  29. Laghari M., Mirjat M.S., Hu Z., Fazal S., Xiao B., Hu M., Chen Z., Guo D. Effects of biochar application rate on sandy desert soil properties and sorghum growth. Catena, 2015, 135: 313-320 CrossRef
  30. Rizhiya E.Ya., Mukhina I.M., Vertebniy V.E., Horak J., Kononchuk P.Yu., Khomyakov Yu.V. Soil enzymatic activity and nitrous oxide emission from light-textured spodosol amended with biochar. Sel'skokhozyaistvennaya biologiya [Agricultural Biology], 2017, 52(3): 464-470 CrossRef
  31. Qayyum M.F., Steffens D., Reisenaue H.P. Schubert S. Kinetics of carbon mineralization of biochars compared with wheat straw in three soils. Journal of Environmental Quality, 2012, 41(4): 1210-1220 CrossRef
  32. Blagodatskaya E.V., Semenov M.V., Yakushev A.V. Aktivnost’ i biomassa pochvennykh mikroorganizmov v izmenyayushchikhsya usloviyakh okruzhayushchey sredy [Activity and biomass of soil microorganisms under changing environmental conditions]. Moscow, 2016 (in Russ.).">CrossRef
  33. Zhuravleva A.I., Myakshina T.N., Blagodatskaya E.V. Mikrobiologiya, 2011, 80(2): 207-218 (in Russ.).
  34. Shang W., Razavi B.S., Yao S., Hao C., Kuzyakov Y., Blagodatskaya E., Tian J. Contrasting mechanisms of nutrient mobilization in rhizosphere hotspots driven by straw and biochar amendment. Soil Biology and Biochemistry, 2023, 187: 109212 CrossRef
  35. Shahnazarova V.Yu., Orlova N.E., Orlova E.E., Bankina T.A., Yakkonen K.L., Rizhiya E.Ya., Kichko A.A. Influence of biochar on the taxonomic composition and structure of prokaryotic communities in agro soddy-podzolic soil. Sel'skokhozyaistvennaya biologiya [Agricultural Biology], 2020, 55(1): 163-173 CrossRef
  36. Guo X.F., Li H.S., Hu Y.M. Effects of biochar on the diversity and community structure of soil fungi in intercropping system. Applied Ecology and Environmental Research, 2019, 17(4): 8817-8834 CrossRef
  37. Tarin M.W.K., Fan L., Xie D. Tayyab M., Rong J., Chen L., Muneer M.A., Zheng Y. Response of soil fungal diversity and community composition to varying levels of bamboo biochar in red soils. Microorganisms, 2021, 9(7): 1385 CrossRef
  38. Gavrilenko E.G., Sus’yan E.A., Anan’eva N.D., Makarov O.A. Pochvovedenie, 2011, 10: 1231-1245 (in Russ.).
  39. Winter K., Beese F. The spatial distribution of soil microbial biomass in a permanent row crop. Biology and Fertility of Soils, 1995, 19(4): 322-326 CrossRef
  40. Maslov M.N., Tokareva O.A., Karavanova E.I., Maslova O.A., Kopeina E.I. Pochvovedenie, 2021, 4: 436-450 CrossRef (in Russ.).
  41. Thiffault E., Hannam K.D., Quideau S.A., Paré D., Bélanger N., Oh S.-W., Munson A.D. Chemical composition of forest floor and consequences for nutrient availability after wildfire and harvesting in the boreal forest. Plant and Soil, 2008, 308: 37-53 CrossRef
  42. Rizhiya E.Ya., Khomyakov Yu.V., Mukhina I.M., Moskvin M.A., Gurova T.A. Agrofizika, 2019, 3: 26-32 CrossRef (in Russ.).
  43. Zheng J., Chen J., Pan G., Liu X., Zhang X., Li L., Bian R., Cheng K., Zheng J. Biochar decreased microbial metabolic quotient and shifted community composition four years after a single incorporation in a slightly acid rice paddy from southwest China. Science of the Total Environment, 2016, 571: 206-217 CrossRef
  44. Zav’yalova N.E., Kovalevskaya N.P., Sharavin D.Yu. Agrokhimiya, 2020, 1: 3-8 CrossRef (in Russ.).
  45. Ma R., Wu X., Liu Z., Yi Q., Xu M., Zheng J., Bian R., Zhang X., Pan G. Biochar improves soil organic carbon stability by shaping the microbial community structures at different soil depths four years after an incorporation in a farmland soil. Current Research in Environmental Sustainability, 2023, 5: 100214 CrossRef

 

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