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Chinarov R.Yu., Bogolyubova N.V., Singina G.N., Lukanina V.A., Rykov R.A., Sermyagin A.A. Association of blood serum biochemical parameters with the follicular pattern of the ovaries and the opu efficiency in donor heifers (Bos taurus L.) of the istoben breed. Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2024, Vol. 59, № 4, p. 704-720.
(how to cite this article)

doi: 10.15389/agrobiology.2024.4.704eng

UDC: 636.2:591.1:591.39

Acknowledgements:
Supported by the Ministry of Science and Higher Education of the Russian Federation within theme no. FGN-2023-0004 (state registration No. 123082900056-2).

 

ASSOCIATION OF BLOOD SERUM BIOCHEMICAL PARAMETERS WITH THE FOLLICULAR PATTERN OF THE OVARIES AND THE OPU EFFICIENCY IN DONOR HEIFERS (Bos taurus L.) OF THE ISTOBEN BREED

R.Yu. Chinarov ✉, N.V. Bogolyubova, G.N. Singina, V.A. Lukanina,
R.A. Rykov, A.A. Sermyagin

Ernst Federal Research Center for Animal Husbandry, 60, pos. Dubrovitsy, Podolsk District, Moscow Province, 142132 Russia, e-mail roman_chinarov@mail.ru (✉ corresponding author), 652202@mail.ru, g_singina@mail.ru, kristybatle@gmail.com, brukw@bk.ru, alex_sermyagin85@mail.ru

ORCID:
Chinarov R.Yu. orcid.org/0000-0001-6511-5341
Lukanina V.A. orcid.org/0000-0003-4744-7873
Bogolyubova N.V. orcid.org/0000-0002-0520-7022
Rykov R.A. orcid.org/0000-0003-0228-8901
Singina G.N. orcid.org/0000-0003-0198-9757
Sermyagin A.A. orcid.org/0000-0002-1799-6014

Final revision received March 18, 2024

Accepted April 12, 2024

 

Increasing the number of cumulus-oocyte complexes (COCs) retrieved per one session of follicle puncture (OPU), as well as improving the quality of COCs in relation to their developmental competence, is a key factor in the effective use of donor cows in programs aimed at in vitro embryo production (IVP). The observed associations between the content of metabolites in the blood of females and in the follicular fluid of the ovary in which oocytes develop suggest that changes in metabolic status due to metabolic disorders can potentially create a suboptimal microenvironment for oocytes, thereby having a negative impact on the quality of oocytes and the success of OPU/IVP programs. In this work, for the first time, the associations of some biochemical parameters of blood serum with the quantity and quality of oocytes and their developmental competence in vitro was found. The aim of this work was to study the associations of the content metabolites and enzymes in blood with the follicular pattern of the ovaries and the efficiency of OPU/IVP in donor heifers. The studies were carried out on mature heifers (n = 6) of the Istoben breed from 22 to 24 months old with a hormonally synchronized estrus cycle. To obtain oocytes, a series of nine consecutive OPU sessions were performed on each donor twice a week (54 sessions in total). Blood sampling for biochemical studies was performed during hormonal treatment and at each OPU session. To characterize nitrogen metabolism, serum levels of total protein, albumins, globulins, urea nitrogen, creatinine, ALT and AST activity were determined. The parameters of carbohydrate-lipid metabolism were controlled by measurements of blood serum glucose, triglycerides, phospholipids, total bilirubin and cholesterol. To assess the status of mineral metabolism, the content of calcium, phosphorus, magnesium, iron, chlorides, and alkaline phosphatase activity in blood serum were determined. The concentrations of all detectable blood metabolites and enzymes were within the reference values, with the exception of alkaline phosphatase, which slightly exceeded the limits. The absence of indices of metabolic processes that "break out" from the norms, as well as insignificant and moderate coefficients of variation, allow exclude the influence of paratypical factors on the animal health status. The two-factor model, considering the Animal and the OPU session, explain 84.2-94.6 % of the variability in the content of the nitrogen metabolites, 86.9 % in glucose concentration, 51.0-94.5 % in lipid metabolites, 42.4-69.2 % in mineral metabolism indices and 99.9 % in alkaline phosphatase activity. The results of the correlation analysis showed the associations of the total number of follicles with the content of certain blood serum metabolites: albumin (r = -0.57, p < 0.01), globulin (r = +0.36, p < 0.01), creatinine (r = -0.31, p < 0.05), cholesterol (r = -0.48, p < 0.05), calcium (r = -0.38, p < 0.01), chlorides (r = -0.30, p < 0.05). Associations of the number of obtained COCs with the content of albumin (r = -0.50, p < 0.01), globulin (r = +0.42, p < 0.01), creatinine (r = -0.31, p < 0.05), cholesterol (r = -0.34, p < 0.05), calcium (r = -0.39, p < 0.01), chlorides (r = -0.30, p < 0.05). Association of creatinine level with the blastocysts rate was established (r = -0.31, p<0.05). We have not found the significant associations between the studied blood parameters and developmental competence of oocytes in vitro. The negative association between creatinine concentration and the percent of oocytes that developed to a blastocyst stage at the trend level was found (r = -0.24, p<0.10). The revealed associations of some blood parameters characterizing the metabolic status of animals with the follicular pattern and the efficiency of OPU in the Istoben heifers under a balanced diet and in the absence of negative effects of paratypical factors on animal health indicate the usefulness of conducting biochemical blood tests before selecting donors for OPU/IVP programs. Further studies on various livestock breeds and considering various technological parameters of OPU will allow formulating metabolic status requirements for donors to provide their most effective use in programs for in vitro production of embryos from OPU oocytes.

Keywords: donor cows, follicle aspiration, Ovum Pick-Up, biochemical blood parameters, oocyte developmental competence, in vitro embryo production.

 

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