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Chernitskiy A.E., Oberiukhtin D.A., Shanina E.P., Kuznetsova Ye.O.,. Oparina O.Yu, Belousov A.I. Metabolic effects of purple potato (Solanum tuberosum L.) dietary juice on rats (a pilot study). Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2024, Vol. 59, № 4, p. 773-786.
(how to cite this article)

doi: 10.15389/agrobiology.2024.4.773eng

UDC: 636.085.8:581.6:577.1

Acknowledgements:
Сarried out within the framework of the state assignments “Creation of competitive, high-yielding varieties of grain, grain-legume, forage, fruit and berry crops and potatoes of world class based on promising genetic resources resistant to bio- and abiotic factors” (0532-2021-0008) and “Development of biological technologies for animal health management and intravital formation of the quality of livestock and poultry products” (0532-2021-0009).

 

METABOLIC EFFECTS OF PURPLE POTATO (Solanum tuberosum L.) DIETARY JUICE ON RATS (a pilot study)

A.E. Chernitskiy ✉, D.A. Oberiukhtin, E.P. Shanina, Ye.O. Kuznetsova, O.Yu. Oparina, A.I. Belousov

Ural Federal Agrarian Research Center, Ural Branch RAS,112a, ul. Belinskogo, Yekaterinburg, 620142 Russia, e-mail cherae@mail.ru (✉ corresponding author), oberuhtindenis@gmail.com, shanina08@yandex.ru, kuzjene@gmail.com, olia91oparina@yandex.ru,
white-knight@mail.ru

ORCID:
Chernitskiy A.E. orcid.org/0000-0001-8953-687X
Kuznetsova Ye.O. orcid.org/0009-0005-6275-3379
Oberiukhtin D.A. orcid.org/0009-0006-1497-5740
Oparina O.Yu. orcid.org/0000-0001-6106-3003
Shanina E.P. orcid.org/0009-0000-5818-3813
Belousov A.I. orcid.org/0000-0002-7838-4126

Final revision received March 07, 2024

Accepted June 06, 2024

 

Potato tubers (Solanum tuberosum L.) are an important food source, containing high concentrations of vitamins C, B vitamins, macroelements (potassium, phosphorus, calcium, magnesium), microelements (iron, iodine, selenium, zinc), complex carbohydrates, and essential amino acids. Varieties with pigmented flesh are rich in carotenoids, tocopherols, lipoic acid, L-tyrosine, and polyphenols. However, the biological effects of potato juice are not well-documented. The aim of this study was to investigate the metabolic effects induced by the inclusion of juice from raw purple-fleshed potato tubers in the diet of white rats. The study included three groups of male non-linear rats, with 6 individuals in each group (age of 9-10 weeks, weight 278.7±5.28 g). Every morning for a month, the experimental group received juice from purple-fleshed potato tubers, while the control groups received juice from regular potatoes and drinking water. Blood samples were obtained at the beginning of the experiment (baseline), after 30 days (treatment), and 60 days (post-treatment). Plasma levels of potassium, sodium, calcium, magnesium, iron, phosphorus, chlorides, bicarbonates, total protein, albumins, globulins, urea, uric acid, creatinine, total cholesterol, triglycerides, glucose, activities of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, γ-glutamyltranspeptidase, total creatine kinase, lactate dehydrogenase, cholinesterase, glutamate dehydrogenase, and lipase were measured using reagent kits (AO Vital Development Corporation, Russia) on a Chem Well-2910 Combi biochemical analyzer (Awaveness Technology, USA). Erythrocyte osmotic resistance as well as malondialdehyde content and total antioxidant status were determined using commercial kits (AO Vector-Best, Russia) on a BioMate 160 UV-Vis spectrophotometer (Thermo Fisher Scientific, the USA). Data were processed using the non-parametric Kruskal-Wallis test with the Dwass-Steel-Critchlow-Fligner post-hoc test (p < 0.05). The following effects occurred during the period of treatment. Compared to baseline values, the plasma content of calcium increased by 10.3 % (p = 0.006), magnesium by 33.3 % (p = 0.004), chloride by 6.63 % (p = 0.018), while a-globulin concentration decreased by 33.9 % (p = 0.006), bicarbonates by 55.9 % (p = 0.005), alkaline phosphatase activity by 41.3 % (p = 0.018). Compared to the negative control, the plasma content of urea increased by 31.2 % (p = 0.019), uric acid by 55.4 % (p = 0.019), potassium by 23.5 % (p = 0.014), potassium-sodium ratio by 22.4 % (p = 0.019) and the index of osmotic resistance of erythrocytes in 0.9 % sodium chloride solution by 74.7 % (p = 0.019), while concentration of total protein decreased by 5,0 % (p = 0,010), α-globulins by 34,3 % (p = 0,019), triglycerides by 26,7 % (p = 0,043), very low density lipoproteins cholesterol by 28,6 % (p = 0,038), and de Ritis coefficient by 20,0 % (p = 0,019). Compared to the positive control, the total antioxidant activity of blood plasma increased by 47.4 % (p = 0.017), while the concentration of α-globulin decreased by 29.1 % (p = 0.049), magnesium by 7.69 % (p = 0.034), aspartate aminotransferase activity by 22.6 % (p = 0.040), alkaline phosphatase activity by 28.9 % (p = 0.019) and de Ritis coefficient by 30.0 % (p = 0.019). The following effects we observed in the post-treatment period. In rats of the experimental group, the plasma iron content increased by 32.4 % (p = 0.010) and 25.8% (p = 0.027) compared to the negative and positive controls, respectively, the concentration of triglycerides decreased by 33.3 % (p = 0.008) and very low density lipoprotein cholesterol by 33.3 % (p = 0.008) compared to the positive control. The results demonstrate that daily consumption of purple potato juice for a month positively affects protein, lipid, and mineral metabolism, as well as the oxidant-antioxidant status of rats.  

Keywords: potato juice, antioxidants, polyphenols, rats, metabolism.

 

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