Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2018, Vol. 53, № 6, p. 1230-1237.
(how to cite this article)

doi: 10.15389/agrobiology.2018.6.1230eng

UDC 636.5:636.082:591.463.1

Acknowledgements:
Supported financially by Russian Science Foundation (grant No. 16-16-04104, study of roosters with transplanted donor spermatogonia) and within the Program of Federal Agency of Scientific Organization (Agreement No. АААА-А18-118021590132-9, study of transgenic roosters produced with the use of lentiviral vectors)

 

CHANGE OF BIOLOGICAL PARAMETERS OF POULTRY SEMEN
AT CRYOPRESERVATION

B.S. Iolchiev, V.A. Bagirov, M.A. Zhilinskiy, N.A. Volkova,
N.A. Zinovieva

L.K. Ernst Federal Science Center for Animal Husbandry,Federal Agency of Scientific Organizations, 60, pos. Dubrovitsy, Podolsk District, Moscow Province, 142132 Russia, e-mail: baylar2@mail.ru (✉ corresponding author), vugarbagirov@mail.ru, naitkin888@mail.ru, natavolkova@inbox.ru, n_zinovieva@mail.ru

ORCID:
Iolchiev B.S. orcid.org/0000-0001-5386-726
Bagirov V.A. orcid.org/0000-0001-5398-8815
Zhilinskiy M.A. orcid.org/0000-0002-5541-9517
Volkova N.A. orcid.org/0000-0001-7191-3550
Zinovieva N.A. orcid.org/0000-0003-4017-6863
The authors declare no conflict of interests

Received September 17, 2018

 

The cryobanks of genetic material are an important element of assisted reproductive technologies. This technology allows more efficient use of genetic material, ensuring to obtain the maximum possible number of offspring for to preserve and restore of rare and endangered species. The most common biomaterial used in programs for the conservation and restoration of the genetic resources of agricultural poultry are spermatozoa. The spermatozoa undergo significant technological treatment during freezing and thawing. Some stages of this cycle are lead to the death of a large part of the cells, damage to their individual organelles or segments. The aim of the research was to study the effect of the freezing and thawing cycle on the biological parameters of spermatozoa in agricultural poultry. The objects of research were adult males of different types of agricultural poultry: roosters Gallus gallus (n = 6), quails Coturnix coturnix (n = 10), guinea fowls Numida meleagris (n = 6), turkeys Meleagris gallopavo (n = 3) and geese Anser anser (n = 4). Qualitative and quantitative indices of freshly received and frozen semen were studied. These indicators include the percentage of mobile spermatozoa, the proportion of spermatozoa with abnormal morphology, the percentage ratio of spermatozoa with abnormal morphology in the head, middle part and flagellum. Sperm was collected 3 times a week. The ejaculates were diluted with the medium for bird sperm dilution (1:1) followed by equilibration of samples at a temperature of 5 °C for 180 minutes. Before cryopreservation, dimethylacetamide was added to the samples as a cryoprotectant by gradually increasing its concentration to 8 %. The samples were frozen in straws (0.25 ml) with an automatic freezer Cryobath Biofreeze BV-65 (CONSARCTIC, Germany). Sperm quality was assessed using software Zoosperm 1.0 (LLC VideoTesT Goss) and Nikon microscope (Nikon Corporation», Japan) equipped with an image input system. To determine the proportion of viable spermatozoa, a supravital staining of a cell smear with 5 % eosin solution was used. The quality indicators of freshly obtained semen complied with the requirements. It was found that the biological value of spermatozoa decreases during the freeze-thaw cycle in all species of poultry conditioned by a decrease in the activity of germ cells. The sperm of the goose was more cryoresistant than the sperm of roosters, quails and turkeys. In geese, after thawing frozen sperm, the content of sperm cells with a straight-forward movement decreased by 30 %, while in roosters, quails and turkeys up to 40-44 %. During semen equilibration, in all poultry species the proportion of live spermatozoa decreased by 7.0-10.6 %. After the freeze-thaw cycle, the proportion of live spermatozoa in the ejaculate decreased compared to the values found for freshly sperm. In roosters, this indicator decreased by 41.6 % with an increase in the percentage of spermatozoa with an abnormal morphology by 22.0 %. In quails these indicators were 43.8 % and 21.8 % and in guinea fowls — 49.1 % and 28.8 %. The most visible disturbances in the morphology of spermatozoa were noted in the flagellum.

Keywords: Gallus gallus L., roosters, Coturnix coturnix L., quails, Numida meleagris L., guinea fowl, Meleagris gallopavo L., turkeys, Anser anser

 

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