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Dinh T.X., Nguyen M.L., Vu D.D., Hoang G.T. High efficient micropropagation of Panax vietnamensis Ha et Grushv. from immature zygotic embryos. Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2021, Vol. 56, № 5, p. 924-933.
(how to cite this article)

doi: 10.15389/agrobiology.2021.5.924eng

UDC: 615.32:576.3/.7.086.83

Acknowledgments:
Funded by the Ministry of Science and Technology of Vietnam in the frame of project «Optimization of a protocol for micropropagation of Ngoc Linh ginseng (Panax vietnamensis) using a bioreactor culture system» (2018-2019)

 

HIGH EFFICIENT MICROPROPAGATION OF Panax vietnamensis Ha et Grushv. FROM IMMATURE ZYGOTIC EMBRYOS

T.X. Dinh1, M.L. Nguyen2 ✉ , D.D. Vu1, G.T. Hoang3 ✉

1Incubation and Support Center for Technology and Science Enterprises, 10000, Hanoi, Vietnam, e-mail dinhxt@gmail.com, dungviae@gmail.com;
2Faculty of Biology and Environmental Science, The University of Da Nang — University of Science and Education, 459 Ton Duc Thang str., Lien Chieu District, 50000, Da Nang, Vietnam, e-mail nmly@ued.udn.vn (✉ corresponding author);
3National Key Laboratory for Plant Cell Biotechnology, LMI RICE-2, Agricultural Genetics Institute, 10000, Hanoi, Vietnam, e-mail nuocngamos@yahoo.com (✉ corresponding author)

ORCID:
Dinh T.X. orcid.org/0000-0002-2200-7655
Vu D.D. orcid.org/0000-0002-8407-8766
Nguyen M.L. orcid.org/0000-0002-0652-891X
Hoang G.T. orcid.org/0000-0001-6891-0121

Received June 9, 2021

 

Despite its significant economic benefit as medicinal plant, cultivation of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) has not been enlarged due to limited source of planting material. Even though considerable research efforts to develop tissue culture systems have been made, micropropagation of Ngoc Linh ginseng is still considered difficult. Here, a micropropagation protocol was established for Ngoc Linh ginseng through somatic embryogenesis from immature zygotic embryos. Direct somatic embryogenesis from immature zygotic embryos was obtained at a rate of 44.33 % on Schenk and Hildebrandt (SH) basal medium with 7 % sucrose. Secondary somatic embryogenesis with proliferation coefficient of 26.71 was induced in liquid-shake culture for 4 week in 1/2 SH medium with 0.1 mg/l kinetin and 0.1 mg/l thidiazuron. The SH medium supplemented with 1 mg/l a-naph-thalenacetic acid, 0.5 mg/l benzyladenine and 5 mg/l gibberellic acid stimulated germination of somatic embryos and early formation of microrhizomes. On two next 10-week cultures on SH medium with 0.5 mg/l a-naphthalenacetic acid, 1 mg/l benzyladenine and 4 % sucrose, micropropagated plantlets were produced with well-developed root system and thickened microrhizome with a dormant bud. The acclimatization of micropropagated plantlets were successfully accomplished with survival rate of 91.67 % when using the soil mixing forest humus with sand in a ratio of 1:2. An efficient protocol developed for Ngoc Linh ginseng micropropagation from immature zygotic embryos comprised five steps, including embryogenesis induction, proliferation of somatic embryos, germination of somatic embryos, development of plantlets, and acclimatization of plantlets.

Keywords: Panax vietnamensis Ha et Grushv., Vietnamese ginseng, Ngoc Linh ginseng, in vitro culture, immature zygotic embryo, somatic embryogenesis, somatic embryo germination, microrhizome, micropropagation.

 

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