Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2011, № 5, p. 45-50.

UDC 633.111.1:581.142:631.52:577.21:57.08

POLYMORPHISM OF MICROSATELLITE LOCUS CAMS-336 IN PEPPER VARIETIES AND CLOSELY RELATED SPECIES

E.A. Snigir’^{1, 2}, O.N. Pyshnaya², E.Z. Kochieva^{1, 3}, N.N. Ryzhova¹

The polymorphism of microsatellite locus CAMS-336 was investigated in 45 pepper varieties of native and foreign selection, and also in variants of closely related cultivated species of the Сapsicum frutescens, C. chinense, C. baccatum. The authors revealed 6 alleles of this locus, distinguished in length, and determined the frequency of its occurrence. The unique alleles for some varieties and species were isolated and the PIC quantities for complete set of variants were calculated. The allelic variability was confirmed by sequencing, which permitted to determine the accurate nucleotide sequence and the size of revealed alleles of microsatellite locus CAMS-336. In addition, the sequencing of this locus permits to determine the mechanism of appearance of new B and D alleles, notably point substitution of T for A. The obtained data suggests the possibility of the use of microsatellite locus CAMS-336 for issue passports to the native varieties of C. аnnuum L.

Keywords: SSR analysis, Сapsicum аnnuum, cultivar fingerprinting.

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