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doi: 10.15389/agrobiology.2021.4.718eng

UDC: 636.93:619:616-093/-098(470.26)

 

EPIZOOTIC MONITORING OF PROTOZOOZES IN THE FUR FARMS OF THE KALININGRAD REGION (2018-2020)

Yu.E. Kuznetsov1 , L.M. Belova1, N.A. Gavrilova1, N.V. Kuznetsova1,
A.B. Muromcev2, A.Yu. Efremov3

1Saint-Petersburg State University of Veterinary Medicine, 5, ul. Chernigovskaya, St. Petersburg, 196084 Russia, e-mail Fish2017@yandex.ru (✉ corresponding author), larissabelova2010@yandex.ru, nadezhda.gavrilova65@mail.ru, n.kuznetcova2018@mail.ru;
2Kaliningrad Branch of the St. Petersburg State Agrarian University, 10, ul. Sovetskaya, Polessk, Kaliningrad Province, 238630Russia, e-mail muromtsev.a@mail.ru;
3Kaliningrad Institute for Retraining Agribusiness Personnel, 2, ul. Molodoi Gvardii, Kaliningrad, 236038 Russia, e-mail alexandrdr1990@gmail.com

ORCID:
Kuznetsov Yu.E. orcid.org/0000-0001-9095-7049
Kuznetsova N.V. orcid.org/0000-0002-3149-1557
Belova L.M. orcid.org/0000-0003-4473-1940
Muromcev А.B. orcid.org/0000-0002-2456-7023
Gavrilova N.A. orcid.org/0000-0001-5651-5976
Efremov A.Yu. orcid.org/0000-0003-3325-8490

Received February 4, 2021

 

Parasitic diseases are widespread in fur-bearing animals, especially in minks. Coccidiidoses occupy a special place among invasive diseases, as they often occur without any symptoms and in some cases are not timely diagnosed. Despite the mild clinical manifestation of invasion, it causes serious damage to animal health and significant economic damage to fur-bearing animal farms. After analyzing the literature data, it became clear that the distribution of eimerioses of fur-bearing animals in the Kaliningrad region has not been studied well enough. In particular, there is no information about the prevalence rates, invasion intensity, and age dynamics of the mink eimeriosis in the fur farms of the region. The purpose of this work was to study the epizootic situation on mink (Mustela vison,Linnaeus, 1761, Neovison vison Schreber, 1777) coccidiidoses in fur-bearing animal farms in the Kaliningrad region. Investigations were performed in three fur-bearing animal farms of the Kaliningrad region. The species composition of protozoa in minks was determined by the morphological features of coccidia and by deep sequencing of the 18S rDNA V4 region. OTUs (operational taxonomic units) revealed by the bioinformatic analysis were used to establish the taxonomic affiliation of pathogens which confirmed the results of light microscopy. In analyzing the obtained results and choosing research methods, the age, sex of the animals, as well as housing and feeding conditions were taken into account. Young minks of 5-6 months and adult livestock, 1-2 year-old females and males, were surveyed. In total, 561 animals were examined in three farms, including farm 1 — 273 minks (198 young animals, 75 adults — 33 males and 42 females); farm 2 — 160 minks (68 young animals, 92 adults — 44 males and 48 females), and farm 3 — 128 minks (28 young animals, 100 adults — 44 males and 56 females). In all the farms surveyed, we found the protozoan of the Eimeriidae family. The greatest prevalence rates (56 %) and the widest variety of coccidia species occurred in the animals of farm 1 under the invasion of two eimeria, Eimeria vison and E. furonis, and two isospores, Isospora laidlawi and I. eversmanni (the I. eversmanni we have earlier identified in minks in the Kaliningrad region for the first time). In all fur farms in the Kaliningrad region, eimerioses more often occur as mono invasions. In mixed invasions, 68.55 % were two-parasite invasions, 23.67 % were three-parasite invasions. Young minks are more susceptible to eimeriidoses than adults. In the animals of the current year of birth, E. vison (18.36 %), I. laidlawi (16.32 %), and E. vison + I. laidlawi (11.90 %) prevailed.

Keywords: Mustela vison, Neovison vison, minks, Eimeria vison, Eimeria furonis, Isospora laidlawi, Isospora eversmanni, mono invasion, mixed invasion, invasion extensity, invasion intensity, fur farms, Kaliningrad region.

 

REFERENCES

  1. Anikieva L.V., Anikanova V.S. V sbornike: Problemy ekologicheskoi fiziologii pushnykh zverei [In: Problems of the ecological physiology of fur animals. Vol. 3]. Petrozavodsk, 2004, vyp. 3: 161-170 (in Russ.).
  2. Poloz S.V. Aktual'nye problemy patologii sel'skokhozyaistvennykh zhivotnykh, 2000, 2: 403-405 (in Russ.).
  3. Gerasimchik V.A. Kishechnye parazitozy pushnykh zverei (etiologiya, epizootologiya, patogenez, diagnostika, terapiya i profilaktika). Avtoreferat doktorskoi dissertatsii [Intestinal parasitosis of fur animals (etiology, epizootology, pathogenesis, diagnosis, therapy, and prevention). DSc Thesis]. Minsk, 2008 (in Russ.).
  4. Kuzovleva L.V. Veterinariya. Referativnyi zhurnal, 2002, 1: 343 (in Russ.).
  5. Vershinin I.I. Koktsidiozy zhivotnykh i ikh differentsial'naya diagnostika [Animal сoccidiosis and their differential diagnosis]. Ekaterinburg, 1996 (in Russ.).
  6. Svanbaev S.K. Prosteishie — vozbuditeli boleznei zhivotnykh Kazakhstana [Protozoa as the causative agents of animal diseases in Kazakhstan]. Alma-Ata, 1967 (in Russ.).
  7. Safiullin R.T. Rossiiskii parazitologicheskii zhurnal, 2008, 2: 84-99 (in Russ.).
  8. Wilcox J.J.S. Something for everyone: a review of «The biology and identification of the Coccidia (Apicomplexa) of carnivores of the world». The American Midland Naturalist, 2019, 181(1): 143-145 CrossRef
  9. Kuznetsov Yu.E. Sovremennaya nauka: aktual'nye problemy i puti ikh resheniya, 2015, 1(14): 48-50 (in Russ.).
  10. Chriél M., Hansen M.S., Petersen H.H., Holm T. Coocidiose hos mink: En undervurderet sygdom? Dansk Pelsdyravl, 2016, 2016(4): 38-38.
  11. Klopfer U., Neuman M. A note on coccidiosis in minks. Refuah Veterinath, 1970, 3(27): 122-124.
  12. Duszynski D.W., Kvicerova J., Seville R.S. The biology and identification of the Coccidia (Apicomplexa) of carnivores of the world. Academic Press, 2018 CrossRef
  13. Nukerbaeva K.K. Vestnik sel'skokhozyaistvennoi nauki Kazakhstana, 1983, 2: 86-88 (in Russ.).
  14. Petersen H.H., Yang R., Chriél M., Hansen M.S., Ryan U.M. Morphological and molecular characterisation of Eimeria vison-like oocysts (ApicomplexaEimeriidae) in farmed mink (Neovison vison) in Denmark. Parasitol. Res.,2018, 117(9): 2933-2939 CrossRef
  15. Yi-Fan C., Le Y., Yin D., Jiang-Hui B. Emendation of 2 Isospora species (Apicomplexa: Eimeriidae) infecting the steppe polecat, Mustela eversmanii Lesson, 1827, in China, to the genus Cystoisospora (Apicomplexa: Sarcocystidae). Comp. Parasitol., 2012, 79: 147-152 CrossRef
  16. Petersen H.H., Yang R., Chriél M., Hansen M.S., Ryan U.M. Morphological and molecular characterization of Eimeria vison-like oocysts (Apicomplexa:Eimeriidae) in farmed mink (Neovison vison) in Denmark. Parasitol. Res., 2018, 117(9): 2933-2939 CrossRef
  17. Sledge D.G., Bolin S.R., Lim A., Kaloustian L.L. Outbreaks of severe enteric disease associated with Eimeria furonis infection in ferrets (Mustela putorius furo) of 3 densely populated groups. Journal of the American Veterinary Medical Association 2011, 239(12): 1584-1588 CrossRef
  18. Kuznetsov Yu.E. Parazitozy pushnykh zverei v khozyaistvakh Severo-Zapadnogo regiona Rossiiskoi Federatsii (mery bor'by i profilaktika). Doktorskaya dissertatsiya [Parasitosis of fur-bearing animals in the farms of the North-West region of the Russian Federation (control measures and prevention). DSc Thesis]. St. Petersburg, 2020 (in Russ.).
  19. Gerasimchik V.A., Zybina O.Yu. Uchenye zapiski UO VGAVM, 2010, 46(1-1): 101-104 (in Russ.).
  20. Belova L.M., Gavrilova N.A., Pudovkin D.N., Tokarev A.N., Kuznetsov Yu.E. Zhidkost' dlya diagnostiki ootsist koktsidii, tsist balantidii i zhiardii, yaits gel'mintov raznykh klassov, kleshchei, nasekomykh, ikh otdel'nykh stadii razvitiya. Pat. RU 2472154 C2 (RF) MPK G01N33/48 A61D99/00. FGBOU VO «SPBGAVM» (RF). № 2010153464/13. Zayavl. 27.12.10. Opubl. 01.10.13. Byul. № 1 [Liquid for the diagnosis of coccidial oocysts, balantidia and giardia cysts, helminth eggs of different classes, ticks, insects, their individual stages of development. Patent RU 2472154 C2 (RF) MPK G01N33/48 A61D99/00 FGBOU VO «SPBGAVM» (RF). № 2010153464/13. Appl. 27.12.10. Publ. 01.10.13. Bull. № 1] (in Russ.).
  21. Belova L.M., Ryazantseva L.T., Gavrilova N.A., Kuznetsov Yu.E., Petrova M.S. «Chashka Petri» Pat. RU № 180046 U1 (RF) MPK: C12M 1/22, FGBOU VO SPBGAVM (RF) № 2017139607.Zayavl. 14.11.2017. Opubl. 31.05.2018 g. Byul. № 23 [«Petri dish» Patent RU № 180046 U1 (RF) MPK: C12M 1/22, FGBOU VO «SPBGAVM» (RF) № 2017139607. Appl. 14.11.2017. Publ. 31.05.2018. Bull. № 23] (in Russ.).
  22. Pellerdy L.P. Coccidia and coccidiosis. Budapest, 1974.
  23. Kuznetsov Y.E., Engashev S.V., Engasheva E.S., Nikonov I.N., Kuznetsova N.V. Microbial community studying of the dogs’ gastrointestinal tract by the T-RFLP molecular genetic method and assessing the natural resistance of animals. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2018, 9(5): 1652-1660.
  24. Carreno R.A., Barta J.R. An Eimeriid origin of isosporoid coccidia with Stieda bodies as shown by phylogenetic analysis of small subunit ribosomal RNA gene sequences. Journal of Parasitology, 1999, 85(1): 77-83.
  25. Kuznetsov Yu.E., Belova L.M., Gavrilova N.A., Mkrtchyan M.E., Sidorenko K.A., Muromtsev A.B. Peculiarities of diagnostics and pathomorphology of eimeriidoses in the mink farms of the northwestern region of the Russian Federation. Sel'skokhozyaistvennaya biologiya [Agricultural Biology], 2020, 55(2): 378-393 CrossRef
  26. Carreno R.A., Schnitzler B.E., Jeffries A.C., Tenter A.M., Johnson A.M., Barta J.R. Phylogenetic analysis of coccidia based on 18S rDNA sequence comparison indicates that Isospora is most closely related to Toxoplasma and Neospora. Journal of Eukaryotic Microbiology, 1998, 45(2): 184-188 CrossRef
  27. Hikosaka K., Nakai Y., Watanabe Y., Tachibana S., Arisue N., Palacpac N.M., Toyama T., Honma H., Horii T., Kita K., Tanabe K. Concatenated mitochondrial DNA of the coccidian parasite Eimeria tenella. Mitochondrion,2011, 11(2): 273-278 CrossRef
  28. Ogedengbe J.D., Hanner R.H., Barta J.R. DNA barcoding identifies Eimeria species and contributes to the phylogenetics of coccidian parasites (Eimeriorina, Apicomplexa, Alveolata). International Journal for Parasitology, 2011, 41(8): 843-850 CrossRef
  29. Pastor A.R. Investigating enteric coccidiosis in the black-footed (Mustela nigripes) and domestic ferret (Mustela putorius furo). PhD. University of Guelph, 2017.
  30. Cavalier-Smith T. Gregarine site-heterogeneous 18S rDNA trees, revision of gregarine higher classification, and the evolutionary diversification of Sporozoa. European Journal of Protistology, 2014, 50(5): 472-495 CrossRef
  31. Wang Q.G., Garrity M., Tiedje J.M., Cole J.R., Naïve Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Applied and Environmental Microbiology, 2007, 73(16):  5261-5267 CrossRef
  32. Klockiewicz M., Jakubowski T., Janecka E., Długosz E. Preliminary epidemiological survey of infections by intestinal parasites in selected mink farms in Poland. Medycyna weterynaryjna, 2013, 69(7): 444-447.

 

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