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Fomichev Yu.P., Bogolyubova N.V., Romanov V.N., Kolodina E.N. Comparative assessment of natural feed additives for functional effects on the digestive processes in the rumen of sheep (Ovis aries). Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2020, Vol. 55, № 4, p. 770-783.
(how to cite this article)

doi: 10.15389/agrobiology.2020.4.770eng

UDC: 636.32/.38:591.132:636.084

Acknowledgements:
Supported financially from the Ministry of Education and Science of the Russian Federation within a support for fundamental research, state registration number NIOKR AAAA-A18-118021590136-7

 

COMPARATIVE ASSESSMENT OF NATURAL FEED ADDITIVES FOR FUNCTIONAL EFFECTS ON THE DIGESTIVE PROCESSES IN THE RUMEN OF SHEEP (Ovis aries)

Yu.P. Fomichev, N.V. Bogolyubova, V.N. Romanov, E.N. Kolodina

Ernst Federal Science Center for Animal Husbandry,60, pos. Dubrovitsy, Podolsk District, Moscow Province, 142132 Russia, e-mail urij.fomichev@yandex.ru, 652202@mail.ru (corresponding author ✉), romanoff-viktor51@yandex.ru, kolodin77@mail.ru

ORCID:
Fomichev Yu.P. orcid.org/0000-0003-0213-5526
Romanov V.N. orcid.org/0000-0002-3542-5370
Bogolyubova N.V. orcid.org/0000-0002-0520-7022
Kolodina E.N. orcid.org/0000-0002-4017-3390

Received April 7, 2020

In ruminant husbandry, ergotropics, fungal cultures, modifiers, antioxidants, enzymes, and other feed additives with various biological properties help practitioners to create optimal conditions for ruminal microorganisms to grow and to digest various feed substrates of the diet. This paper first compares functional effects of bioactive natural substances (dihydroquercetin, organic iodine, micellar calcium, spirulina, and shungite) on the rumen digestion in Romanov sheep. The work aimed to investigate the peculiarities of the influence of natural feed additives different in their biological properties on fermentative and microbiological processes in the rumen of sheep. Dietary trials were performed in the conditions of the physiological yard, the Ernst Federal Science Center for Animal Husbandry, with Romanov sheep (Ovis aries). In 2014, 12 animals aged 4-5 months were assigned to four dietary treatments: a basal diet without supplementation (BD, the control animals), BD + shungite (0.3 % dry matter), BD + shungite (0.9 % dry matter), and BD + shungite (1.5 % dry matter). In 2018-2019, six fistulated sheep aged 2 years were fed according to two schemes. The first scheme of dietary treatment was as follows: BD for equalization, BD (control), dihydroquercetin (DHQ) (100 mg·head-1·day-1) + organic iodine (OI) (1.05 mg·head-1·day-1), BD for equalization, and DHQ (100 mg·head-1·day-1), each period lasted 14 days. In the second scheme, micellar calcium (M-Ca) injected into the rumen through a fistula (50 µl·head-1·day-1) was used instead of DHQ + OI, and spirulina (1,25 g·head-1·day-1, the dry powder mixed with 23.75 g filler) instead of DHQ. In all tests, at the end of each period, 3 hours after feeding, rumen contents were sampled to assess pH, redox potential (ROP), oxidization, total amount of volatile fatty acids (VFA), concentration of ammonia nitrogen, amylolytic activity, the biomass of ruminal protozoa and bacteria, and the microbial profiles. It was found that DHQ and spirulina have the most pronounced effect on ruminal pH, with a 12.7 and 9.5 % increase in the chyme pH, respectively. All dosages of shungite and M-Ca provide higher production of volatile fatty acids (VFA), the 20.6; 27.2 (р < 0.05), 22.8 and 6.6 % of the controls. DHQ + OI, DHQ and spirulina, in contrast, depress VFA production to 93.5, 81.2 and 81.8 % levels of the controls. The dietary additives, except for DHQ + OI, decrease the chyme level of ammonia, which was the greatest for DHQ and M-Ca, by 24.1 % at p < 0.01 and 26.3 %, respectively. Shungite also depresses the chyme ammonia concentration by 18.7 (p < 0.05), 14.9 (p < 0.05) and 9.4 % compared to the control. Dietary DHQ + OI, on the contrary, increases this indicator by 16.5 %. Dietary spirulina, M-Ca and DHQ + OI resulted in the 6.1, 1.7 and 1.1 % increase in the chyme amylolytic activity, respectively, the dietary shungite causes its significant decrease, by 16.8-19.9 %, while the influence of DHQ is the least with a 7.3 % decrease. It was shown that the rumen fermentation affects counts and species composition of the microbiota. The greatest increase in the chyme microbial biomass results from dietary shungite and depends on the mineral dosage. Dietary shungite increases the overall number of microorganisms 2 times (p < 0.01), by 47.4 % (p < 0.05) and 25.2 %, respectively, mainly due to infusoria, and changes the percentage ratio between infusoria and bacteria. DHQ + OI, DHQ and M-Ca also lead to an increase in the chyme microbial biomass but to a much lesser extent as compared to the shungite, i.e. to 14.6, 2.2 and 1.8 % of the control, respectively. Dietary spirulina depresses the total number of microorganisms by 14.3 %. The study traces a positive relationship between the effects of the studied natural feed additives on the rumen microbiocenosis and the ammonia/VFA ratio of, which can characterize the rate of feed conversion to microbial protein.

Keywords: feed additives, shungite, dihydrouridine, organic iodine, spirulina, Romanov sheep, rumen, fermentation, chyme, amylase, volatile fatty acids, ammonia, microbiota.

 

REFERENCES

  1. Russell J.B., Rychlik J.L. Factors that alter rumen microbial ecology. Science, 2001, 292(5519): 1119-1222 CrossRef
  2. Agarwal N., Kamra D.N., Chaudhary L.C. Rumen microbial ecosystem of domesticated ruminants. In: Rumen microbiology: from evolution to revolution. A. Puniya, R. Singh, D. Kamra (eds.). Springer, New Delhi. 2015 CrossRef
  3. Russell J.B., Rychlik, J.L. Factors that alter rumen microbial ecology. Science, 2001, 292(5519): 1119-1122 CrossRef
  4. Krehbie C.R. Invited review: applied nutrition of ruminants: fermentation and digestive physiology. The Professional Animal Scientist, 2014, 30(2): 129-139 CrossRef
  5. Chernyshev N.I., Panin I.G., Shumskii N.I., Grechishnikov V.V. Antipitatel'nye faktory kormov. Spravochnaya kniga [Anti-nutritional factors of feed]. Voronezh, 2013 (in Russ.).
  6. Ward J. Probiotic yeast for optimal rumen balance. All About Feed, 2017, 25(8): 24-25.
  7. Maksimov R.T. Veterinariya sel'skokhozyaistvennykh zhivotnykh, 2008, 3: 64-65 (in Russ.).
  8. Bogolyubova N., Korotky V., Zenkin A., Ryzhov V., Buryakov N. Digestion and metabolism indices of sheep when using activated charcoal supplement. OnLine Journal of Biological Sciences, 2017, 17(2): 121-127 CrossRef
  9. Artem’eva O.A., Pereselkova D.A., Fomichev Yu.P. Dihydroquercetin, the bioactive substance, to be used against pathogenic microorganisms as an alternative to antibiotics. Agricultural Biology [Sel'skokhozyaistvennaya biologiya], 2015, 50(4): 513-519 CrossRef
  10. Aliev M.M., Gulieva K.A. Vestnik APK Stavropol'ya, 2018, 1(29): 57-59 (in Russ.).
  11. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA), Turck D., Bresson J.-L., Burlingame B., Dean T., Fairweather-Tait S., Heinonen M., Hirsch-Ernst K., Mangelsdorf I., McArdle H.J., Naska A., Neuhäuser-Berthold M., Nowicka G., Pentieva K., Sanz Y., Siani A., Sjodin A., Stern M., Tome D., Vinceti M., Willatts P., Engel K.-H., Marchelli R., Pöting A., Poulsen M., Schlatter J., Gelbmann W., Loveren H.V. Scientific opinion on taxifolin-rich extract from Dahurian Larch (Larix gmelinii). EFSA Journal, 2017, 15(2): 4682 CrossRef
  12. Fomichev Yu.P., Nikanova L.A., Dorozhkin V.I., Torshkov A.A., Romanenko A.A., Es'kov E.K., Semenova A.A., Gonotskii V.A., Dunaev A.V., Yaroshevich G.S., Lashin S.A., Stol'naya N.I. Digidrokvertsetin i arabinogalaktan — prirodnye bioregulyatory: primenenie v sel'skom khozyaistve i pishchevoi promyshlennosti. Monografiya [Dihydroquercetin and arabinogalactan, the natural bioregulators: application in agriculture and food industry. Monograph]. Moscow, 2017 (in Russ.).
  13. Aghwan Z.A., Sazili A.Q., Kadhim K.K., Alimon A.R., Goh Y.M., Adeyemi K.D. Effects of dietary supplementation of selenium and iodine on growth performance, carcass characteristics and histology of thyroid gland in goats. Animal Science Journal, 2015, 87(5): 690-696 CrossRef
  14. Aghwan Z.A, Sazili A.Q., Alimon A.R., Goh Y.M., Hilmi M.A. Blood haematology, serum thyroid hormones and glutathione peroxidase status in Kacang goats fed inorganic iodine and selenium supplemented diets. Asian-Australasian Journal of Animal Sciences, 2013, 26(11): 1577-1582 CrossRef
  15. Xu J., Liu X.-L., Yang X.-F., Guo H.-L., Zhao L.-N., Sun X.-F. Supplemental selenium alleviates the toxic effects of excessive iodine on thyroid. Biological Trace Element Research, 2011, 141: 110-118 CrossRef
  16. Rakhmanin Yu.A., Stekhin A.A., Yakovleva G.V. Biofizika vody [Biophysics of water]. Moscow, 2016 (in Russ.).
  17. Zatsepina O.V., Stekhin A.A., Yakovleva G.V., P'yanzina I.P. Gigiena i sanitariya, 2013, 5: 37-39 (in Russ.).
  18. Ametov A.S., Doskina E.V., Kapustina N.V. Endokrinologiya, 2016, 4(17): 65-72 (in Russ.).
  19. Shibil'skis P. Aktivirovannaya voda [Activated water]. Available: https://water-ionizer.ru/useful-information/aktivirovannaya-voda. Accessed: 16.03.2019 (in Russ.).
  20. Rekomendatsii po ispol'zovaniyu ionizirovannoi vody [Recommendations for the use of ionized water]. Available: https://akvalife.club/ru/rekomendatsii-po-primeneniyu-ionizirovannoj-vody.html. Accessed: 16.03.2019 (in Russ.).
  21. Becker E.W. Microalgae for human and animal nutrition. In: Handbook of microalgal culture: applied phycology and biotechnology, second edition. Richmond A., Hu Q. (eds.). Blackwell Publishing Ltd., 2013 CrossRef
  22. Kedik S.A., Yartsev E.I., Gul'tyaeva N.V. Spirulina — pishcha XXI veka [Spirulina as a food of the XXI century]. Moscow, 2006 (in Russ.).
  23. Kedik S.A., Yartsev E.I., Sakaeva I.V., Zhavoronok E.S., Panov A.V. Biofarmatsevticheskii zhurnal, 2011, 3(3): 3-10 (in Russ.).
  24. Zaitsev V.V., Tarabrin V.V., Voishcheva E.A. Vestnik meditsinskogo instituta "REAVIZ": reabilitatsiya, vrach i zdorov'e, 2011, 1: 17-21 (in Russ.).
  25. Holman B.W.B., Malau-Adul A.E.O. Spirulinaas a livestock supplement and animal feed. Journal of Animal Physiology and Animal Nutrition, 2012, 97(4): 615-623 CrossRef
  26. Rimkus M., Simkus A., Syvys R., Birutis S. Dry powdery fodder additive, supplement or fodder containing algae spirulina platensis. WO/2010/106468 MKI3 A23K 1/00 2006.01, A23K 1/02 2006.01, C12N 1/12 2006.01. SPILA, UAB (LT). № PCT/IB2010/051039. Decl. 10.03.2010. Publ. 23.09.2010.
  27. Vetrov S.I., Lenkova N.I., Gorbatkina I.E. Shungit. Rossiiskii mineral [Shungite. Russian mineral]. Moscow, 2011 (in Russ.).
  28. Andrievsky G.B., Bruskov V.I., Tykhomyrov A.A., Gudkov S.V. Peculiarities of the antioxidant and radioprotective effects of hydrated C60 fullerene nanostructures in vitro and in vivo. Free Radical Biology and Medicine, 2009, 47(6): 786-793 CrossRef
  29. Xiao Y., Wiesner M.R. Characterization of surface hydrophobicity of engineered nanoparticles. Journal of Hazardous Materials, 2012, 215-216: 146-151 CrossRef
  30. Zhang Y., Matsuo, Y., Li C.-Z., Tanaka H., Nakamura E. A scalable synthesis of methano[60]fullerene and congeners by the oxidative cyclopropanation reaction of silylmethylfullerene. Journal of the American Chemical Society, 2011, 133(21): 8086-8089 CrossRef
  31. Ponomarev A.P., Podkolzin I.V., Amelin V.G. Nanotekhnologii: nauka i proizvodstvo, 2012, 4(11): 48-55 (in Russ.).
  32. Bogolyubova N.V., Romanov V.N., Devyatkin V.A., Kalinin Yu.K. Veterinariya i kormlenie, 2014, 5: 88-90 (in Russ.).
  33. Tremasova A.M. Materialy nauchno-prakticheskoi konferentsii «Innovatsiya v sovremennoi farmakologii» [Proc. Conf. “Innovation in modern pharmacology”]. Kazan'-Moscow, 2012: 180-181 (in Russ.).
  34. Nasrollahi S.M., Zali A., Ghorbani G.R., Moradi S.M., Heydari S.A.M. Variability in susceptibility to acidosis among high producing mid-lactation dairy cows is associated with rumen pH, fermentation, feed intake, sorting activity, and milk fat percentage. Animal Feed Science and Technology, 2017, 228: 72-82 CrossRef
  35. Dijkstra J., Ellis J.L., Kebread E., Strathe A.B., López S., France J., Bannink A. Ruminal pH regulation and nutritional consequences of low pH. Animal Feed Science and Technology, 2012, 172(1-2): 22-33 CrossRef
  36. Malekkhanhi M., Tahmasbi A.M., Naserian A.A., Danesh-Mesgaran M., Kleen J.L., AlZahal O., Ghaffari M.H. Effects of supplementation of active dried yeast and malate during subacute ruminal acidosis on rumen fermentation, microbial population, selected blood metabolites, and milk production in dairy cows. Animal Feed Science and technology, 2016, 213: 29-43 CrossRef
  37. Abdullah M.A.M., Farghaly M.M., Youssef I.M.I. Effect of feeding Acacia nilotica pods to sheep on nutrient digestibility, nitrogen balance, ruminal protozoa and rumen enzymes activity. Journal of Animal Physiology and Animal Nutrition, 2018, 102(3): 662-669 CrossRef
  38. Benchaar C., McAllister T.A., Petit H.V., Chouinard P.Y. Whole flax seed and oil supplementation of dairy cows fed high-forage or high-concentrate diets: effect on digestion/ruminal fermentation characteristics, protozoal population and milk fatty acid profile. Animal Feed Science and technology, 2014, 198: 117-129 CrossRef
  39. Cone J.W., Becker P.M. Fermentation kinetics and production of volatile fatty acids and microbial protein by starchy feedstuffs. Animal Feed Science and technology, 2012, 172 (1-2): 34-41 CrossRef
  40. Ghaffari M.H., Tahmasbi A.M., Khorvash M., Naserian A.-A., Ghaffari A. H., Valizadeh H. Effects of pistachio by-products in replacement of alfalfa hay on populations of rumen bacteria involved in biohydrogenation and fermentative parameters in the rumen of sheep. Journal of Animal Physiology and Animal Nutrition, 2013, 98(3): 578-586 CrossRef
  41. Khezri A., Dayani O., Tahmasbi R. Effect of increasing levels of wasted date palm on digestion, rumen fermentation and microbial protein synthesis in sheep. Journal of Animal Physiology and Animal Nutrition, 2016, 101(1), 53-60 CrossRef
  42. Ariko T., Kass M., Henno M., Fievez V., Kärt O., Kaart T., Ots M. The effect of replacing barley with glycerol in diet of dairy cows on rumen parameters and milk fatty acid profile. Animal Feed Science and Technology, 2015, 209: 69-78 CrossRef
  43. Qiao G.H., Zhou X.H., Li Y., Zhang H.S., Li J.H., Wang C.M., Lu Y. Effect of several supplemental Chinese herbs additives on rumen fermentation, antioxidant function and nutrient digestibility in sheep. Journal of Animal Physiology and Animal Nutrition, 2011, 96(5): 930-938 CrossRef
  44. Schofield B.J., Lachner N., Le O.T., McNeill D.M., Dart P., Ouwerkerk D., Hugenholtz P., Klieve A.V. Beneficial changes in rumen bacterial community profile in sheep and dairy calves as a result of feeding the probiotic Bacillus amyloliquefaciens H57. Journal of Applied Microbiology, 2018, 124(3): 855-866 CrossRef
  45. Bogolyubova N., Korotky V., Zenkin A., Romanov V., Ryzhov V. Assessing efficiency of the coniferous energy supplement in the diet of dairy cows for maintaining productive health. Asian Journal of Pharmaceutical and Clinical Research, 2017, 10(10): 117-120 CrossRef
  46. Hosoda K., Matsuo M., Miyaji M., Matsuyama H., Maeda H., Ohta H., Kato H., Nonaka K. Fermentative quality of purple rice (Oryza sativa L.) silage and its effects on digestibility, ruminal fermentation and oxidative status markers in sheep: A preliminary study. Grassland Science, 2012, 58(3): 161-169 CrossRef
  47. Váradyová Z., Mravčáková D., Holodová M., Grešáková Ľ., Pisarčíková J., Barszcz M., Taciak M., Tuśnio A., Kišidayová S., Čobanová K. Modulation of ruminal and intestinal fermentation by medicinal plants and zinc from different sources. Journal of Animal Physiology and Animal Nutrition, 2018, 102(5): 1131-1145 CrossRef
  48. Makkar H.P.S., Tran G., Heuze V., Giger-Reverdin S., Lessire M., Lebas F., Ankers P. Seaweeds for livestock diets: a review. Animal Feed Science and Technology, 2016, 212: 1-17 CrossRef

 

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