Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2018, Vol. 53, № 4, p. 735-742.
(how to cite this article)

doi: 10.15389/agrobiology.2018.4.735eng

UDC 636.1:636.082:591.463.15:591.11:575

ASSOCIATED CONNECTION OF ERYTHROCITARY
ANTIGENS WITH CHARACTERISTICS OF STALLION
SEMEN AFTER CRYOCONSERVATION

A.V. Tkachеv^{1, 2}, O.L. Tkacheva³, V.I. Rossokha³

¹National University of Pharmacy, 53, vul. Pushkinskaya, Kharkov, Ukraine 61002, e-mail sasha_sashaola@mail.ru (✉ corresponding author);
²Gorin Belgorod State Agricultural University, 1, ul. Vavilova, pos. Maiskii, Belgorod Region, Belgorod Province, 308503 Russia, e-mail sasha_sashaola@mail.ru (✉ corresponding author);
³Institute of Animal Science of National Academy of Agrarian Sciences of Ukraine, 3,vul. 7-i Gvardeiskoi Armii, Kharkov, Ukraine 61120, e-mail tkacheva.olga2017@gmail.com, rossokha.v@ukr.net

ORCID:
Tkachеv A.V. orcid.org/0000-0002-7721-5742
Rossokha V.I. orcid.org/0000-0001-8709-7535
Tkachеva O.L. orcid.org/0000-0002-5573-6117
The authors declare no conflict of interests

Received July 10, 2017

Cryopreservation of animal sperm is the most important way to preserve endangered breeds and species. This is of particular relevance in the horse breeding of Russia and Ukraine given the critical decrease in the livestock of pedigree animals to 1.3 million and up to 300 thousand heads, respectively. For example, in Ukraine only 3 out of 12 officially registered breeds have the minimum required number of breeding stallions and mares. Therefore, the increase in the characteristics of stallion sperm and the improvement of the ability to predict the effectiveness of cryopreservation are of great scientific and practical importance. This article presents the results of the studies on the associated dependence of erythrocyte antigens A, C, D and K of the blood groups of horses of Ukrainian selection. The associated relationship of the efficiency of cryopreservation of stallion sperm has been revealed depending on the antigenic characteristics of erythrocytes according to A, C, D, and K blood group systems. It has been shown that the obtaining of the average parameters of stallion spermatozoa motility and survival after cryopreservation of 2.5 points and 4 hours, respectively, was accompanied by the presence of alleles of erythrocyte antigens bcm/cgm, bcm/de, bcm/dg, bcm/dk, cegm/cgm, cegm/d, cegm/dg, cegm/dk, cgm/cgm, cgm/d, cgm/dg, de/d, de/dk, dk/d, dk/de, dk/dk of the blood group D system. High semen characteristics after cryopreservation, i.e. the motility more than 4 points and survival spermatozoa more than 4 hours were obtained from the stallions with alleles of erythrocyte antigens bcm/d, cgm/de, dg/dk of the blood group D. The degree of the influence of erythrocyte antigens of the blood group D on the cryoresistance of the stallion semen is 32.5 % (P < 0.001), on the motility of the thawed semen — 18.2 % (P < 0.01), and on the survival rate of the thawed spermatozoa — 25.2 % (P < 0.001). The absence of the blood group A antigens in the stallions was accompanied by a significant increase in the biological characteristics of the semen after cryopreservation. In the absence of erythrocyte antigens of the blood group A, the activity of the sperm in stallions was higher by 0.51 points (P < 0.05) as compared to the control, by 1.36 points (P < 0.01) as compared to the carriers of а/-, by 0.17 points as compared to the stallions with аd/-. It has been shown that the presence of а/-of the blood group C contributed to a significant (P < 0.05) impairment of the activity (by 0.33 points), of the survival (by 0.78 hours) and of the semen durability (by 6.26 %) as compared to the stallions in which there were no alleles of this blood group system. If the antigens of the blood group K were not  inherited by stallions, the activity of the thawed sperm was 0.35 points higher (P < 0.05) than in the carriers of а/- of the K system, and by 0.40 points (P < 0.05) higher than in the control stallions in which the antigenic profile of erythrocytes was not determined. It has been found out that the degree of the influence of the alleles of the blood group K on the activity of spermatozoa after thawing was 1.4 % (P < 0.05), on the semen durability — 2.0 % (P < 0.05), on the absolute value of durability — 1.5 % (Р < 0.05), on the spermatozoa preservation —  1.2 % (Р < 0.05). The generalized dispersion analysis of the obtained data has shown that the degree of the influence of the antigenic characteristics of erythrocytes on sperm cryoresistance in the stallions of Ukrainian selection was 38.7 % (P < 0.001) for blood group D, 1.7 % (P < 0.05) for blood group A, 16.6 % (Р < 0.01) for blood group С, and 12.9 % (Р < 0.01) for blood group К.

Keywords: erythrocyte antigens, alleles of systems of blood groups, cryopreservation of semen, horses, stallions.

Full article (Rus)

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