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Shpanev А.М., Denisyuk Е.S., Shilova O.A, Semenov K.N., Panova G.G. Carbon and silica nanostructures in the protection of spring barley from diseases in the North-West Russia. Bioelectrochemical systems based on the electroactivity of plants and microorganisms in the root environment (review). Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2022, Vol. 57, № 3, p. 441-459.
(how to cite this article)

doi: 10.15389/agrobiology.2022.3.441eng

UDC: 632:546.26:631.8:581.1(470.2)

Acknowledgements:
Supported financially by the Agrophysical Research Institute from the Fundamental Scientific Research Program of the State Academies of Sciences for 2013-2020, stage No. 0667-2019-0013 in terms of ensuring the conduct of vegetation and field experiments and with partial financial support from the Russian Foundation for Basic Research No. 15-29-05837ofi_m in terms of creating biologically active carbon and silica-sol nanocompositions and studying their effect on the physiological state of plants

 

CARBON AND SILICA NANOSTRUCTURES IN THE PROTECTION OF SPRING BARLEY FROM DISEASES IN THE NORTH-WEST RUSSIA

А.М. Shpanev1 ✉, Е.S. Denisyuk1, O.A. Shilova2, K.N. Semenov3, G.G. Panova1

1Agrophysical Research Institute, 14, Grazhdanskii prosp., St. Petersburg, 195220 Russia, e-mail ashpanev@mail.ru (✉ corresponding author), mitek1604@yandex.ru, gaiane@inbox.ru;
2Institute of Silicate Chemistry RAS (ICHS), St. Petersburg, nab. Makarova, 2, 199034 Russia, e-mail olgashilova@bk.ru;
3Pavlov First Saint Petersburg State Medical University, St. Petersburg, ul. L’va Tolstova, 6-8, 197022 Russia, e-mail semenov1986@yandex.ru

ORCID:
Shpanev A.M. orcid.org/0000-0003-4346-318X
Semenov K.N. orcid.org/0000-0003-2239-2044
Denisyuk Е.S. orcid.org/0000-0003-0663-1316
Panova G.G. orcid.org/0000-0002-1132-9915
Shilova O.A. orcid.org/0000-0002-3856-9054

Received May 19, 2022

Spring barley (Hordeum vulgare L.) is the main grain fodder crop, annually occupying about 40 % of the sown area in the North-West Russia. In recent years, there has been a clear interest in the world and domestic science to use of nanomaterials and nanotechnologies in plant protection, which is due to their unique properties and high efficiency at low concentrations. In this work, for the first time, the effect of carbon and silica sol nanocompositions on seed infection, damage to spring barley plants by root rot and leaf diseases is shown. It was determined that a stronger protective effect was manifested when using nanocompositions on the spring barley variety Ataman with a longer growing season and more susceptible to major diseases. For the first time, an additive effect has been established that enhances the protective functions of chemical or biological fungicides with the possibility of reducing their dosage when combined with nanocomposites in the treatment of seeds and vegetative plants. Our goal was to study the effectiveness of new compositions based on carbon and silica sol nanomaterials in protecting spring barley from diseases in the North-West Russia. The studies were carried out at the experimental base of the Menkovsky branch of the Agrophysical research institute (Gatchinsky District, Leningrad Province) in 2017-2018. At the first stage of research in 2017, the effectiveness of two promising nanocompositions for the protection of spring barley from root rot and leaf diseases was studied. Two experiments were carried out on Leningradsky and Ataman varieties of spring barley with different vegetation periods: on the treatment of seed material and vegetative plants with nanocompositions. The silica sol composition of NKteos was synthesized according to the original sol-gel technology based on acid hydrolysis followed by polycondensation of tetraethyl ester of orthosilicic acid or tetraethoxysilane, with the addition of macro- and microelements salts solutions and dopants — a charge of detonation nanodiamond doped with boron, or a titanium dioxide in the form of anatase to the sol. Preparation of a nanocomposition based on fullerene derivatives with methionine or threonine was carried out by dissolving microelement compounds in water and adding 0.001 % (for seed treatment) or 0.00001 % (for foliar treatment) solution of the amino acid derivative of C60 fullerene with threonine or with methionine. Experiment variants also included the combined use of nanocompositions with chemical and biological fungicides, as well as fungicides with silicon-containing chelated microfertilizer. Grain contamination with phytopathogens was determined using nutrient media. The development of root rot was t assessed in the phases of germination, tillering, budding and heading, leaf diseases — in the beginning of barley earing, then in 10, 20 and 30 days. At the second stage of research in 2018, the effectiveness of the technological scheme for the use of new nanocompositions in the protection of spring barley of the Leningradsky variety from diseases was evaluated. The experiment included two blocks: the treatment with nanocompositions of seeds, the treatment of seeds and vegetative plants. It is shown that the studied nanocompositions in their pure form turned out to be ineffective in protecting spring barley from root rot and leaf diseases. The decrease in the development of root rot on the early ripe variety Leningradsky did not exceed 5.3 %, on the variety Ataman it was 15.3-57.7 % (p < 0.05). The development of the main disease of the crop — helminthosporium spots on the two upper leaves of barley plants of the Leningradsky variety decreased by 16-22 %, of the Ataman variety — by 20-42 % (p < 0.05). The results of seed treatment allow us to assume that the effect of the silica sols composition is longer, since it extended to the development of helminthosporium leaf spots (decrease in damage by 7.5-15.4 %, p < 0.05 compared to control) and is due to the ability to activate plant metabolism and immunity. The effect of the nanocomposition based on the fullerene C60-methionine derivative is more apparent due to a decrease in seed infection and primary signs of infection during the emergence of barley seedlings. The most effective for the protection of spring barley from root and leaf diseases was the combined treatment of seeds with a silica sol nanocomposition and the chemical fungicide Insure™ Perform, KS, followed by a triple treatment of vegetative plants with a nanocomposition based on a C60-threonine derivative and a single treatment with the chemical fungicide Zantara, CE. Reducing the dose of a chemical preparation is advisable only if a weak manifestation of the disease is expected. High biological and economic efficiency, comparable to the result of fungicidal treatment with 100 % application rate of the preparation, was ensured by the combined use of silicon containing chelate microfertilizer SCM-G and fungicide (50 % application rate), as well as nanocomposition based of the C60 fullerene amino acid derivatives with methionine and fungicide (50 % application rate).

Keywords: Hordeum vulgare L., spring barley, root rot, leaf diseases, plant protection products, fungicides, nanomaterials, fullerene C60, amino acid derivatives, C60with methionine, C60 with threonine, silica sol, tetraethoxysilane. dopants, charge of detonation nanodiamond, titanium dioxide, anatase.

 

REFERENCES

  1. Frew A., Weston L.A., Reynolds O.L., Gurr G.M. The role of silicon in plant biology: a paradigm shift in research approach. Annals of Botany, 2018, 121(7): 1265-1273 CrossRef
  2. Polischuk S.D., Churilov G.I., Churilov D.G., Borychev S.N., Byshov N.V., Koloshein D.V., Cherkasov O.V. Biologically active nanomaterials in production and storage of arable crops. International Journal of Nanotechnology, 2019, 16(1-3): 133-146 CrossRef
  3. Kalenska S., Novytska N., Stolyarchuk T., Kalenskyi V., Garbar L., Sadko M., Shutiy O., Sonko R. Nanopreparations in technologies of plants growing. Agronomy Research, 2021, 19(Special Issue 1): 795-808 CrossRef
  4. Fedorenko V.F., Bukhlagin D.S., Golubev I.G., Nemenushchaya L.A. Povyshenie urozhaynosti sel’skokhozyaystvennykh kul’tur primeneniem nanotekhnologiy. Nauchnoe izdanie [Increasing crop yields using nanotechnology. Scientific publication]. Moscow, 2013 (in Russ.).
  5. Polishchuk S.D., Nazarova A.A., Stepanova I.A., Kutskir M.V., Churilov D.G. Nanotekhnika, 2014, 1(37): 72-81 (in Russ.).
  6. Zabegalov N.V., Dabakhova E.V. Dostizheniya nauki i tekhniki APK, 2011, 12: 22-24 (in Russ.).
  7. Khoroshilov A.A., Pavlovskaya N.E., Borodin D.B., Yakovleva I.V. A nanosilicon preparation is superior to a biological preparation and a chemical preparation in activity towards photosynthetic productivity and yield parameters of spring wheat. Sel'skokhozyaistvennaya biologiya [Agricultural Biology], 2021, 56(3): 487-499 CrossRef
  8. Semina S.A., Gavryushina I.V., Nikulina E.V. Agrokhimicheskiy vestnik, 2020, 4: 62-66 (in Russ.).
  9. Semenov K.N., Meshcheriakov A.A., Charykov N.A., Dmitrenko M.E., Keskinov V.A., Murin I.V., Panova G.G., Sharoyko V.V., Kanash E.V., Khomyakov Yu.V. Physico-chemical and biological properties of C60-l-hydroxyproline water solutions. RSC Advances, 2017, 7: 15189-15200 CrossRef
  10. Panova G.G., Serebryakov E.B., Semenov K.N., Charykov N.A., Shemchuk O.S., Andrusenko E.V., Kanash E.V., Khomyakov Y.V., Shpanev A.M., Dulneva L.L., Podolsky N.E., Sharoyko V.V. Bioactivity study of the C60-L-threonine derivative for potential application in agriculture. Journal of Nanomaterials, 2019, 2019: 2306518 CrossRef
  11. Mahadeva Swamy H.M., Asokan R. Bacillus thuringiensis as ‘Nanoparticles’ — a perspective for crop protection. Nanoscience & Nanotechnology-Asia, 2013, 3(1): 102-105 CrossRef
  12. Cooke J., Leishman M.R. Consistent alleviation of abiotic stress with silicon addition: a metaanalysis. Functional Ecology, 2016, 30(8): 1340-1357 CrossRef
  13. Abdel-Haliem M.E.F., Hegazy H.S., Hassan N.S., Naguib D.M. Effect of silica ions and nanosilica on rice plants under salinity stress. Ecological Engineering,2017, 99: 282-289 CrossRef
  14. Piotrovskiy L.B., Litasova E.V., Dumpis M.A. Obzory po klinicheskoy farmakologii i lekarstvennoy terapii, 2019, 17(2): 5-15 CrossRef (in Russ.).
  15. Kresge C.T., Leonowicz M.E., Roth W.J., Vartuli J.C., Beck J.S. Ordered mesoporous molecular sieves synthesized by a liquid-crystal template mechanism. Nature, 1992, 359(6397): 710-712 CrossRef
  16. Beck J.S., Vartuli J.C., Roth W.J., Leonowicz M.E., Kresge C.T., Schmitt K.D., Chu C. T-W., Olson D.H., Sheppard E.W., McCullen S.B., Higgins J.B., Schlenker J.L. A new family of mesoporous molecular sieves prepared with liquid crystal templates. J. Am. Chem. Soc., 1992, 114(27): 10834-10843 CrossRef
  17. Shilova O.A. Synthesis and structure features of composite silicate and hybrid TEOS-derived thin films doped by inorganic and organic additives. J. Sol-Gel Sci. Technol.,2013, 68(3): 387-410 CrossRef
  18. Panova G.G., Ktitorova I.N., Skobeleva O.V., Sinjavina N.G., Charykov N.A., Semenov K.N. Impact of polyhydroxy fullerene (fullerol or fullerenol) on growth and biophysical characteristics of barley seedlings in favourable and stressful conditions. Plant Growth Regulation, 2016, 79(3): 309-317 CrossRef
  19. Panova G.G., Kanash E.V., Semenov K.N., Charykov N.A., Khomyakov Yu.V., Anikina L.M., Artem’eva A.M., Kornyukhin D.L., Vertebnyy V.E., Sinyavina N.G., Udalova O.R., Kulenova N.A., Blokhina S.Yu. Fullerene derivatives influence production process, growth and resistance to oxidative stress in barley and wheat plants. Sel'skokhozyaistvennaya biologiya [Agricultural Biology], 2018, 53(1): 38-49 CrossRef
  20. Panova G.G., Semenov K.N., Shilova O.A., Kornyukhin D.L., Shpanev A.M., Anikina L.M., Khamova T.V., Artem’eva A.M., Kanash E.V., Charykov N.A., Udalova O.R., Galushko A.S., Zhuravleva A.S., Filippova P.S., Kudryavtsev D.V., Blokhina S.Yu. Agrofizika, 2018, 3: 48-58 (in Russ.).
  21. Shilova, O.A., Khamova, T.V., Panova, G.G., Artem’eva, A.M., Kornyukhin, D.L. Using the sol-gel technology for the treatment of barley. Glass Physics and Chemistry, 2018, 44(1): 25-32 CrossRef
  22. Shilova O.A., Khamova T.V., Panova G.G., Kornyukhin D.L., Anikina L.M., Artemyeva A.M., Udalova O.R., Galushko A.S., Baranchikov A.E. Synthesis and research of functional layers based on titanium dioxide nanoparticles and silica sols formed on the surface of seeds of chinese cabbage. Russian Journal of Applied Chemistry, 2020, 93(1): 25-34 CrossRef
  23. Shvidchenko A.V., Eidelman E.D., Vul' A.Ya., Kuznetsov N.M., Stolyarova D.Yu., Belousov S.I., Chvalun S.N. Colloids of detonation nanodiamond particles for advanced applications. Advances in Colloid and Interface Science, 2019, 268: 64-81 CrossRef
  24. Schrand A.M., Ciftan Hens S.A., Shenderova O.A. Nanodiamond particles: properties and perspectives for bioapplications. Critical Reviews in Solid State and Materials Sciences,2009, 34(1-2): 18-74 CrossRef
  25. Dolmatov V.Yu., Ozerin A.N., Kulakova I.I., Bochechka O.O., Lapchuk N.M., Myllymäki V., Vehanen A. Detonation nanodiamonds: new aspects in the theory and practice of synthesis, properties and applications. Russian Chemical Reviews,2020, 89(12): 1428-1462 CrossRef
  26. Isakova A.A., Safonov A.V., Aleksandrovskaya A.Yu., Galushko T.B., Indenbom A.V., Spitsyn B.V. Fizikokhimiya poverkhnosti i zashchita materialov,2017, 53(2): 137-140 (in Russ.).
  27. Panyuta O., Belava V., Fomaidi S., Kalinichenko O., Volkogon M., Taran N. The effect of pre-sowing seed treatment with metal nanoparticles on the formation of the defensive reaction of wheat seedlings infected with the eyespot causal agent. Nanoscale Research Letters, 2016, 11: 92 CrossRef
  28. Pandey S., Giri K., Kumar R., Mishra G., Raja Rishi R. Nanopesticides: opportunities in crop protection and associated environmental risks. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 2016, 88(1): 1287-1308 CrossRef
  29. Arkhipov M.V., Danilova T.A., Sinitsyna S.M. V sb.: Nauchnoe obespechenie razvitiya proizvodstva zerna na Severo-Zapade Rossii [In: Scientific support for the development of grain production in the North-West of Russia]. St. Petersburg, 2014: 4-15 (in Russ.).
  30. Metodicheskie ukazaniya po registratsionnym ispytaniyam fungitsidov v sel’skom khozyaystve [Guidelines for registration testing of fungicides in agriculture]. St. Petersburg, 2009 (in Russ.).
  31. Rogozhnikova E.S., Shpanev A.M. Vestnik zashchity rasteniy, 2016, 3(89): 140-142 (in Russ.).
  32. Shpanev A.M., Denisyuk E.S. Efficiency of microbial preparations based on Bacillus subtilis and Trichoderna harzianum for the protection of spring barley from diseases in Northwestern Russia. AppliedBiochemistryandMicrobiology, 2020, 56(9): 930-939 CrossRef
  33. GOST 12044-93. Mezhgosudarstvennyy standart. Semena sel’skokhozyaystvennyy kul’tur. Metody opredeleniya zarazhennosti boleznyami [GOST 12044-93. Interstate standard. Seeds of agricultural crops. Methods for determining disease infestation]. Moscow, 1993 (in Russ.).
  34. Anikina L.M., Panova G.G. Kremniysoderzhashchee khelatnoe mikroudobrenie i sposob ego polucheniya. Patent na izobretenie 2515389 (RF). Zayavl. 29.08.2012 g. Opubl. 10.05.2014. Byul. № 13 [Silicon-containing chelate microfertilizer and method for its production. Patent for invention 2515389 (Russian Federation). Appl. 29.08.2012. Publ. 10.05.2014. Bull. No. 13](in Russ.).
  35. Janmohammadi M., Sabaghnia N., Ahadnezhad A. Impact of silicon dioxide nanoparticles on seedling early growth of lentil (Lens culinaris Medik.) genotypes with various origins. Agriculture & Forestry, 2015, 61(3): 19-33 CrossRef
  36. Qi M., Liu Y., Li T. Nano-TiO2 improve the photosynthesis of tomato leaves under mild heat stress. Biological Trace Element Research, 2013,156: 323-328 CrossRef
  37. Khodakovskaya M.V., Kim B.-S., Kim J.N., Alimohammadi M., Dervishi E., Mustafa T., Cernigla C.E. Carbon nanotubes as plant growth regulators: effects on tomato growth, reproductive system, and soil microbial community. Nano-micro Small, 2013, 9(1): 115-123 CrossRef
  38. Kim Y.-H., Khan A.L., Waqas M., Lee I.-J. Silicon regulates antioxidant activities of crop plants under abiotic-induced oxidative stress: a review. Frontiers in Plant Science, 2017,8: 510 CrossRef
  39. Jo Y.-Ki, Kim B.H., Jung G. Antifungal activity of silver ions and nanoparticles on phytopathogenic fungi. Plant Disease, 2009, 93(10): 1037-1043 CrossRef
  40. Zhang Y., Qi G.Y., Yao L., Huang L., Wang J., Gao W. Effects of metal nanoparticles and other preparative materials in the environment on plants: from the perspective of improving secondary metabolites. Journal of Agricultural and Foot Chemistry, 2022, 70(4): 916-933 CrossRef
  41. Ayoub H.A., Khairy M., Elsaid S., Rashwan F.A., Abdel-Hafez H.F. Pesticidal activity of nanostructured metal oxides for generation of alternative pesticide formulations. Journal of Agricultural and Foot Chemistry, 2018, 66(22): 5491-5498 CrossRef

 

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