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Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2019, Vol. 54, № 3, p. 548-556.
(how to cite this article)

doi: 10.15389/agrobiology.2019.3.548eng

UDC: 633.913:581.142

Acknowledgments:
The authors wish to thank Mr. Hui Han for his seed test assistance.
The work was performed in the framework of the VIR state assignment on the topic No. 0662-2019-0001.
Supported by the Team Promoting Project of Heilongjiang Academy of Sciences under grant number 2014ST06, and by the Technology Application Research and Development Project of Harbin program under the contract number 2014AE4AE053

 

METHODOLOGICAL STUDY ON SELECTION OF CONDITIONS FOR IMPROVING Taraxacum kok-saghyz Rodin SEED GERMINATION

G. Shen1, 2, F. Chen2, W. Wang1, F. Zheng2, D. Wei2, L. Li2, N.G. Kon’kova3

1The Ministry of Education Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, e-mail shen19772@163.com (✉ corresponding author);
2Institute of Natural Resources and Ecology, Heilongjiang Academy of Sciences, Harbin, 150040, e-mail shen19772@163.com;
2Federal Research Center the Vavilov All-Russian Institute of Plant Genetic Resources, Federal Agency for Scientific Organizations, 42-44, ul. Bol’shaya Morskaya, St. Petersburg, 190000 Russia, e-mail n.konkova@vir.nw.ru (✉ corresponding author)

ORCID:
Shen G. orcid.org/0000-0001-5320-6465
Wei D. orcid.org/0000-0002-8610-4921
Chen F. orcid.org/0000-0002-8890-9782
Li L. orcid.org/0000-0002-4423-6934
Wang W. orcid.org/0000-0003-0465-686X
Kon’kova N.G. orcid.org/0000-0002-4920-3904
Zheng F. orcid.org/0000-0002-8966-5230

Received February 27, 2018

 

Currently, Kok Sagyz is considered to be the most promising source of natural rubber. In many countries significant investments in the development of rubber production technologies based on the use of this plant are being made. Ensuring a high percentage of rapid and uniform germination is important for Kok Sagyz cultivation. In this research we have obtained results significantly extending the idea of temperature as a factor affecting the characteristics of Kok Sagyz seeds germination, which is important for this rubber plant reproduction practice. The aim of our study was to determine a practical and simple method to improve the germination of Taraxacum kok-saghyz seed. An orthogonal array design L9 (34)] was used to optimize three factors: concentration of KMnO4 solution (0.07 %, 0.1 %, and 0.2 %), soaking time (1, 2, and 4 h), and germination temperature (4/17, 17, and 23 °С). Germination parameters, including germination percentage, T50 (time taken for 50 % of seeds to germinate), germination index, and vigor index, were evaluated. Using analysis of variance, the optimum conditions for germination were determined, i.e. 2 h of soaking time in 0.07 % KMnO4 solution and a germination temperature of 23 °С. The optimum conditions were subsequently validated. Under the optimized conditions, we achieved a germination percentage of 71 %, a T50 value of 11 days, and germination and vigor indices of 7.22 and 14.40, respectively. Moreover, we found that in addition to moisture, temperature was the main factor influencing the germination of T. kok-saghyz seeds. Solution composition and concentration and soaking time had little or no effect on germination.

Keywords: germination percentage, germination uniformity, KMnO4, potassium permanganate, Taraxacum kok-saghyz Rodin, Russian dandelion.

 

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