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Brandorf A.Z., Shestakova A.I., Larkina E.O. Lapynina E.P. Effect of acaricide treatment on body weight and reproductive characteristics of drones of the Prioksky breed type of Central Russian honeybees (Apis mellifera Linnaeus, 1758) Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2023, Vol. 58, № 2, p. 345-354.
(how to cite this article)

doi: 10.15389/agrobiology.2023.2.345eng

UDC: 638.12:57.017.53:612.616.2

Acknowledgements:
Supported by the Ministry of Science and Higher Education of the Russian Federation (state order No. 0470-2019-0001)

 

EFFECT OF ACARICIDE TREATMENT ON BODY WEIGHT AND REPRODUCTIVE CHARACTERISTICS OF DRONES OF THE PRIOKSKY BREED TYPE OF CENTRAL RUSSIAN HONEYBEES (Apis mellifera Linnaeus, 1758)

A.Z. Brandorf ✉, A.I. Shestakova, E.O. Larkina, E.P. Lapynina

Federal Beekeeping Research Centre, 22, ul, Pochtovaya, Rybnoye, Ryazan Province, 391110 Russia, e-mail gordenchuk@mail.ru (✉ corresponding author), anastasiya_07.83@mail.ru, alenaelena98@yandex.ru, elena.p56@yandex.ru

ORCID:
Brandorf A.Z. orcid.org/0000-0003-0535-6578
Larkina E.O. orcid.org/0000-0002-5405-8721
Shestakova A.I. orcid.org/0000-0002-7311-9307
Lapynina E.P. orcid.org/0000-0002-6473-1393

Final revision received August 18, 2022
Accepted  

In modern beekeeping, there is a mass death of bee colonies, one of the causes of which is varroosis. To reduce the negative impact of varroosis on the life of a bee colony, acaricides are widely used. However, the acaricidal preparations negatively affect the reproductive performance of drones. Various reports note their ambiguous effect on some the development of individuals in a bee family, including drones, and, consequently, economically useful traits (honey and wax productivity, queen egg production, resistance to diseases). In this work, for the first time, we obtained data that acaricidal preparations of amitraz, fluvalinate and thymol + oxalic acid negatively affect the reproductive performance of honeybee drones of the Central Russian breed and cause a deterioration in sperm quality. The aim of the work was to study the effect of acaricidal preparations on the fertility, deformation and concentration of spermatozoa of honey bee drones of the Prioksky breed type of the Central Russian breed, as well as on their body weight. The work was carried out at the experimental apiary of the Federal Beekeeping Research Centre (Rybnoye, Ryazan Province, spring-summer 2021). Colonies of Central Russian bees (Apis mellifera Linnaeus, 1758) of the Prioksky breed type were assigned to four groups, three bee colonies each: group I was not subjected to treatments (control), groups II-IV were treated. The degree of Varroa destructor infestation was 1-2 % in all groups. After the appearance of one-day-old drone brood, each test group was treated with one of three acaricidal drugs to combat varroosis. An amitraz-based drug («Sichuan Wangshi Animal Health Co., Ltd.», China; hazard class 3, SanPiN 1.2.25.84-10) was used in group II, a fluvalinate-based drug («Shanxi Zhenxing Fish & Bees Medicine Industry Co., Ltd.», China; hazard class 3, SanPin 1.2.25.84-10) in group III, and a drug containing oxalic acid («Shandong Deshang Chemical Co., Ltd.», China) and thymol («Hunan Insen Biotech Co., Ltd.», China) (hazard class 4, SanPin 1.2.25.84-10) in group IV. Preparations containing the active ingredients amitraz and thymol + oxalic acid were sprayed onto drone brood in 2 repetitions with 7-day interval. The fluvalinate-containing strips were placed on both sides of the drone brood frame. The impact of antivarroal drugs was assessed based on sperm quality parameters and body weight of drones at the age of 26-30 days. Sperm was collected by artificially stimulating endophallus eversion in mature drones aged 26-30 days. The concentration of spermatozoa, motility, viability, morphology (defects and abnormalities) were assessed. When assessing the viability of spermatozoa by fluorescent microscopy, fluorochromes Hoechst 33258 (Pan-Eco, Russia) and PI (Khimmed, Russia) were used with a biological luminescent light-emitting diode microscope MICROMED 3LYUM LED (OOO Observational Instruments, Russia) with 400× magnification. To determine the deformation of the heads of spermatozoa, rapid differentiated staining with a set of reagents Diahim-Diff-Quick (OOO "ABRIS + NPF", Russia) was used. Our results show that the treatment of bee colonies with acaricides affects the weight of drones. The decrease in body weight was significant when using fluvalinate and oxalic acids + thymol preparations, where the maximum weight of drones was 10-20 mg less (p < 0.05). It was found that sperm quality parameters decrease after treatment with acaricides. The viability of spermatozoa decreased by 1.3 % on average, sperm concentration decreased 2.2 times, and the number of spermatozoa with abnormal head morphology increased 1.3 times. Therefore, acaricides should be used only for medicinal purposes when varroasis is confirmed. The frequency of use should depend on the degree of invasion in order to reduce the negative impact of drugs on the reproductive function of drones.

Keywords: Apis mellifera, honey bee, drone, sperm quality, spermatozoa viability, spermatozoa morphology, acaricidal preparations, amitraz, fluvalinate, thymol, oxalic acid.

 

REFERENCES

  1. Abrol D.P. Honeybee and crop pollination. In: Pollination biology: biodiversity conservation and agricultural production. Springer Science, Business Media, 2012: 85-110.
  2. Afanas’ev V.I. Еkonomika, trud, upravlenie v sel’skom khozyaystve, 2018, 2: 76-81 (in Russ.).
  3. Kayode L., Lizette D., Johnson R.M., Siegfried B.D., Ellis M.D. Effect of amitraz on queen honey bee egg and brood development. Mellifera, 2014, 14: 33-40.
  4. Lar’kina E.O., Svishchuk D.V., Lapynina E.P. Vestnik RGATU, 2021, 13(1): 23-30 CrossRef (in Russ.).
  5. Rosenkranz P., Aumeier P., Ziegelmann B. Biology and control of Varroa destructor. Journal of Invertebrate Pathology, 2010, 103: 96-119 CrossRef
  6. Cameron S.A., Sadd B.M. Global trends in bumble bee healt. Annual Review of Entomology, 2020, 65: 209-232 CrossRef
  7. Zattara E.E. Aizen M.A. Worldwide occurrence records suggest a global decline in bee species richness. One Earth, 2021, 4(1): 114-123 CrossRef
  8. Evans J.D., Schwarz R.S. Bees brought to their knees: microbes affecting honey bee health. Trends in Microbiology, 2011, 19(12): 614-620 CrossRef
  9. Messan K., Rodriguez Messan M., Chen J., DeGrandi-Hoffman G., Kang Y. Population dynamics of Varroa mite and honeybee: effects of parasitism with age structure and seasonality. Ecological Modelling,2021,440: 109359 CrossRef
  10. Smodiš Škerl M.I., Kmecl V., Gregorc A. Exposure to pesticides at sublethal level and their distribution within a honey bee (Apis mellifera) colony. Bull. Environ. Contam. Toxicol., 2010, 85(2): 125-128 CrossRef
  11. Grankin N.N., Guseva Yu.Yu. Nauka-2020, 2018, 9(25): 53-57 (in Russ.).
  12. Al-Waili N., Salom K., Al-Ghamdi A., Ansari M.J. Antibiotic, pesticide, and microbial contaminants of honey: human health hazards. Scientific World Journal, 2012: 930849 CrossRef
  13. Aufauvre J., Biron D.G., Vidau C., Fontbonne R., Roudel M., Diogon M., Viguès B., Belzunces L.P., Delbac F., Blot N. Parasite-insecticide interactions: a case study of Nosema ceranae and fipronil synergy on honeybee. Scientific Reports, 2012,2: 326 CrossRef
  14. Pettis J.S., vanEngelsdorp D., Johnson J., Dively G. Pesticide exposure in honey bees results in increased levels of the gut pathogen Nosema. Naturwissenschaften, 2012, 99(2): 153-158 CrossRef
  15. Kairo G., Biron D.G., Abdelkader F.B., Bonnet M., Tchamitchian S., Cousin M., Dussaubat C., Benoit B., Kretzschmar A., Belzunces L.P., Brunet J.-L. Nosema ceranae, fipronil and their combination compromise honey bee reproduction via changes in male physiology. Sci. Rep., 2017, 7: 8556 CrossRef
  16. Terebova S.V., Koltun G.G., Podvalova V.V. Agrarnyy vestnik Primor’ya, 2021, 1(21): 37-43 (in Russ.).
  17. Sammataro D. Global status of honey bee mites. In: Honey bee colony health, challenges and sustainable solutions. D. Sammataro, J.A. Yoder (eds.). Boca Raton, CRC Press, 2012: 37-54.
  18. Isaev Yu.G. Rossiyskiy zhurnal problemy veterinarnoy sanitarii, gigieny i еkologii,2020, 4(36): 507-510 CrossRef (in Russ.).
  19. Burley L.M., Fell R.D., Saacke R.G. Survival of honey bee (Hymenoptera: Apidae) spermatozoa incubated at room temperature from drones exposed to miticides. Journal of Economic Entomology, 2008, 101(4): 1081-1087 CrossRef
  20. Fell R.D., Tignor K. Miticide effects on the reproductive physiology of queens and drones. American Bee Journal, 2001, 141: 888-889.
  21. Ben Abdelkader F. Impact of pesticides on honeybee (Apis mellifera L.) drones. Uludag Bee Journal, 2019, 19(2): 188-194 CrossRef
  22. Shoukry R., Khattaby A., El-Sheakh A., Abo-Ghalia A., Elbanna S. Effect of some materials for controlling varroa mite on the honeybee drones (Apis mellifera L.). Egyptian Journal of Agricultural Research, 2013, 91(3): 825-834.
  23. Tihelka E. Effects of synthetic and organic acaricides on honey bee health: a review. Slov. Vet. Res., 2018, 55(2): 119-40 CrossRef
  24. Bevk D., Kralj J., Čokl A. Coumaphos affects food transfer between workers of honeybee Apis mellifera. Apidologie, 2011, 43: 465-470 CrossRef
  25. Williamson S.M., Baker D.D., Wright G.A. Acute exposure to a sublethal dose of imidacloprid and coumaphos enhances olfactory learning and memory in the honeybee. Apis mellifera. Invertebrate Neuroscience, 2013, 13: 63-70 CrossRef
  26. Chaimanee V., Evans J.D., Chen Y., Jackson C., Pettis J.S. Sperm viability and gene expression in honey bee queens (Apis mellifera) following exposure to the neonicotinoid insecticide imidacloprid and the organophosphate acaricide coumaphos. Journal of Insect Physiology, 2016, 89: 1-8 CrossRef
  27. Ciereszko A., Wilde J., Dietrich G.J., Siuda M., Bąk B., Judycka S., Karol H. Sperm parameters of honeybee drones exposed to imidacloprid. Apidologie, 2017, 48(2): 211-222 CrossRef
  28. Kairo G., Provost B., Tchamitchian S., Ben Abdelkader F., Bonnet M., Cousin M., Sénéchal J., Benet P., Kretzschmar A., Belzunces L.P., Brunet J.L. Drone exposure to the systemic insecticide Fipronil indirectly impairs queen reproductive potential. Scientific Reports, 2016, 6: 31904 CrossRef
  29. Kairo G., Poquet Y., Haji H., Tchamitchian S., Cousin M., Bonnet M., Pelissier M., Kretzschmar A., Belzunces L.P., Brunet J.-L. Assessment of the toxic effects of pesticides on honey bee drone fertility using laboratory and semifield approaches: a case study of fipronil. Environ. Toxicol. Chem., 2017, 36(9): 2345-2351 CrossRef
  30. Rangel J., Fisher II A. Factors affecting the reproductive health of honey bee (Apis mellifera) drones — a review. Apidologie, 2019, 50: 759-778 CrossRef
  31. Ilyasov R.A., Lim S., Lee M.L., Kwon H.W., Nikolenko A.G. Effect of miticides amitraz and fluvalinate on reproduction and productivity of honey bee Apis mellifera. Uludag Bee Journal (Uludağ Arıcılık Dergisi), 2021, 21(1): 21-30 CrossRef
  32. Lazareva L.N. V sbornike: Sbornik NIIR po pchelovodstvu [Collection of NIIR on beekeeping]. Kirov, 2014 (in Russ.).
  33. Rhodes J.W. Semen production in drone honey bees. RIRDC Pub., 2008.
  34. Locke S.J., Peng Y.-S., Cross N.L. A supravital staining technique for honey bee spermatozoa. Physiological Entomology, 1990, 15(2): 187-192.
  35. Smith C.C. Opposing effects of sperm viability and velocity on the outcome of sperm competition. Behavioral Ecology, 2012, 23(4): 820-826 CrossRef
  36. Abdelkader F.B., Çakmak I., Çakmak S.S., Nur Z., Incebiyik E., Aktar A., Erdost H. Toxicity assessment of chronic exposure to common insecticides andbee medications on colony development and drones spermparameters. Ecotoxicology, 2021, 30(1): 806-817 CrossRef

 

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