PLANT BIOLOGY
ANIMAL BIOLOGY
SUBSCRIPTION
E-SUBSCRIPTION
 
MAP
MAIN PAGE

 

 

 

 

doi: 10.15389/agrobiology.2022.2.343reng

UDC: 636.5:591.3:591.05

Acknowledgements:
Supported financially by Russian Foundation for Basic Research, project No. 20-016-00204-а

 

FEATURES OF NITRIC OXIDE METABOLISM IN EMBRYOS OF DIFFERENT BIRD SPECIES AS GENETICALLY DETERMINED SIGN ASSOCIATED WITH MEAT PRODUCTIVITY

V.Yu. Titov1, 2 , A.M. Dolgorukova1, I.I. Kochish2,
O.V. Myasnikova2, I.N. Nikonov2

1Federal Scientific Center All-Russian Research and Technological Poultry Institute RAS, 10, ul. Ptitsegradskaya, Sergiev Posad, Moscow Province, 141311 Russia, e-mail vtitov43@yandex.ru (✉ сorresponding author), anna.dolg@mail.ru;
2Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, 23, ul. Akademika K.I. Skryabina, Moscow, 109472 Russia, e-mail prorector@mgavm.ru, omyasnikova71@gmail.com, ilnikonov@yandex.ru

ORCID:
Titov V.Yu. orcid.org/0000-0002-2639-7435
Myasnikova O.V. orcid.org/0000-0002-9869-0876
Dolgorukova A.M. orcid.org/0000-0002-9958-8777
Nikonov I.N. orcid.org/0000-0001-9495-0178
Kochish I.I. orcid.org/0000-0001-8892-9858

Received December 29, 2021

 

At present, the role of nitric oxide (NO) in embryogenesis, in particular in myogenesis, is widely discussed. Earlier we noted that the main part of nitric oxide synthesized in the avian embryo can accumulate in tissues as part of the so-called NO donor compounds or be oxidized to nitrate. The degree of this oxidation correlates with the meat productivity of adults. This report shows that in broiler embryos, NO is oxidized to nitrate by 90% or more, while in embryos of egg poultry NO oxidation is negligible. That is, the degree of NO oxidation is determined by some features of the embryo tissues rather than NO itself determines these features. Consequently, the degree of NO oxidation in bird embryogenesis is an indicator associated with tissue properties correlating with meat productivity. Since this sign is inherited, it is assumed to be genetically determined. The purpose of this work is to characterize the manifestation and inheritance of the intensity of nitric oxide oxidation and the associated physiological characteristics of embryos in birds of different species.The purpose of this work is to characterize the manifestation and inheritance of the intensity of nitric oxide oxidation and the associated physiological characteristics of bird embryos of different species. The experiments were carried out in a vivarium (Zagorskoye, Sergiev Posad, Moscow Province, 2015-2021). It was shown that in poultry of different breeds characterized by the same degree of NO oxidation the live weight can vary significantly. This is especially evident in hens. The proportion of oxidized NO in the embryo was higher in lines, breeds and crosses obtained as a result of breeding to increase meat productivity. Thus, in the embryos of broilers and meat quails, by the day 7th, more than 90% of embryonic NO is oxidized, in egg forms oxidation was insignificant (several percent), most meat-egg forms occupied an intermediate position according to this index. The analysis of inheritance of the index in the F1 generation in several bird species suggests that this trait is formed due to the expression of various genes that can both promote and counteract its manifestation. Oxidation of NO to nitrate in embryos of both meat and egg forms can be induced by light at the beginning of incubation. In embryos of egg forms, the proportion of oxidized NO can increase up to 60 % under the action of light. Consequently, there is a possibility of oxidation of NO in embryos of both meat and egg forms. Apparently, the mechanism of activation of this process is inherited, which can also be partially induced by light. Further analysis of the inheritance of the intensity of oxidation of embryonic NO in a number of generations will show which genes are associated with the intensity of oxidation of NO. This will allow using this indicator as a highly sensitive marker for the corresponding genes.

Keywords: nitric oxide, NO, NO donors, NO oxidation, trait inheritance, nitrate, myogenesis, Gallus gallus domesticus L., chickens, Coturnix coturnix L., quail, Numida meleagris L., guinea fowl, Struthio camelus L., ostriches.

 

REFERENCES

  1. Li Y., Wang Y., Willems E., Willemsen H., Franssens L., Buyse J., Decuypere E.,  Everaert N. In ovo L-arginine supplementation stimulates myoblast differentiation but negatively affects muscle development of broiler chicken after hatching. Journal of Animal Physiology and Animal Nutrition, 2016, 100: 167-77 CrossRef
  2. Tirone M., Conti V., Manenti F., Nicolosi P., D’Orlando C., Azzoni E., Brunelli S. Nitric oxide donor molsidomine positively modulates myogenic differentiation of embryonic endothelial progenitors. PLoS ONE,2016, 11(10): e0164893 CrossRef
  3. Long J., Lira V., Soltow Q., Betters J., Sellman J., Criswell D. Arginine supplementation induces myoblast fusion via augmentation of nitric oxide production. J. Muscle Res. Cell Motil., 2006, 27: 577-584 CrossRef
  4. Stamler J., Meissner G. Physiology of nitric oxide in skeletal muscle. Physiol. Rev., 2001, 81: 209-237 CrossRef
  5. Ulibarri J., Mozdziak P., Schultz E., Cook C., Best T. Nitric oxide donors, sodium nitroprusside and S-nitroso-N-acetylpencillamine, stimulate myoblast proliferation in vitro. In Vitro Cell Dev. Biol. Anim., 1999, 35(4): 215-218 CrossRef
  6. Anderson J.E. A role for nitric oxide in muscle repair: nitric oxide-mediated activation of muscle satellite cells. Molecular Biology of the Cell, 2000, 11: 1859-1874 CrossRef
  7. Severina I., Bussygina O., Pyatakova N., Malenkova I., Vanin A. Activation of soluble guanylate cyclase by NO donors-S-nitrosothiols, and dinitrosyl-iron complexes with thiol-containing ligands. Nitric Oxide,2003, 8: 155-163 CrossRef
  8. Stalmer J., Singel D., Loscalzo  J. Biochemistry of nitric oxide and its redox-activated forms Science,1992, 258:1898-1902 CrossRef
  9. Rossig L., Fichtlscherer B., Breitschopf K., Haendeler J., Zeiher A., Mulsch A., Dimmeler S. Nitric oxide inhibits caspase-3 by S-nitrosation in vivo J. Biol. Chem.,1999,274(11): 6823-6826 CrossRef
  10. Dimmeler S., Haendeler J., Nehls, M., Zeiher A. Suppression of apoptosis by nitric oxide via inhibition of interleukin-1beta-converting enzyme (ICE)-like and cysteine protease protein (CPP)-32-like proteases. J. Exp. Med., 1997, 85(4): 601-607 CrossRef
  11. Kim Y.-M., Chung H.-T., Simmons R., Billiar T. Cellular non-heme iron content is a determinant of nitric oxide-mediated apoptosis, necrosis, and caspase inhibition. J. Biol. Chem., 2000, 275(15): 10954-10961 CrossRef
  12. Vasudevan D., Bovee R., Tomas D. Nitric oxide, the new architect of epigenetic landscapes. Nitric Oxide,2016, 59: 54-62 CrossRef
  13. Socco S., Bovee R., Palczewski M., Hickok J. Epigenetics: the third pillar of nitric oxide signaling. Pharmacological Research, 2017, 121: 52-58 CrossRef
  14. Vanin A., Borodulin R., Mikoyan V. Dinitrosyl iron complexes with natural thiol-containing ligands in aqueous solutions: synthesis and some physico-chemical characteristics (A methodological review). Nitric Oxide, 2017, 66: 1-9 CrossRef
  15. Vanin A. Dinitrosyl iron complexes with thiol-containing ligands as a “working form” of endogenous nitric oxide. Nitric Oxide, 2016, 54: 15-29 CrossRef
  16. Hickok J.R., Sahni S., Shen H., Arvind A., Antoniou C., Fung L.W., Thomas D. Dinitrosyliron complexes are the most abundant nitric oxide-derived cellular adduct: biological parameters of assembly and disappearance. Free Radic. Biol. Med., 2011, 51(8): 1558-1566 CrossRef
  17. Titov V.Y., Kosenko O.V., Starkova E.S., Kondratov G.V., Borkhunova E.N., Petrov V.A., Osipov A.N. Enzymatic sensor detects some forms of nitric oxide donors undetectable by other methods in living tissues. Bull. Exp. Biol. Med., 2016, 162(1): 107-110 CrossRef
  18. Titov V.Yu., Dolgorukova A.M., Fisinin V.I., Borkhunova Ye.N., Kondratov G.V., Slesarenko N.A., Kochish I.I. The role of nitric oxide (NO) in the body growth rate of birds. World’s Poultry Science Journal, 2018, 74(4): 675-686 CrossRef
  19. Titov V.Yu., Vinnikova E.Z., Akimova N.S., Fisinin V.I. Nitric oxide (NO) in bird embryogenesis: physiological role and ability of practical use. World’s Poultry Science Journal, 2012, 68(1): 83-95 CrossRef
  20. Dolgorukova A.M., Titov V.Yu., Kochish I.I., Fisinin V.I., Nikonov I.N., Kosenko O.V., Myasnikova O.V. The embryonic metabolism of nitric oxide and its interrelation with postembryonic development in chicken (Gallus gallus domesticus L.) and quals (Coturnix coturnix L.). Sel’skokhozyaistvennaya Biologiya [Agricultural biology], 2020, 55(3): 794-803 CrossRef

back

 


CONTENTS

 

 

Full article PDF (Rus)

Full article PDF (Eng)