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Saktaganov N.T., Klivleyeva N.G., Ongarbayeva N.S., Glebova T.I., Lukmanova G.V., Baimukhametova A.M. Study on antigenic relationships and biological properties of swine influenza a/h1n1 virus strains isolated in Northern Kazakhstan in 2018. Sel'skokhozyaistvennaya Biologiya [Agricultural Biology], 2020, Vol. 55, № 2, p. 355-363.
(how to cite this article)

doi: 10.15389/agrobiology.2020.2.355eng

UDC: 636.4:619:578(574.2)

 

STUDY ON ANTIGENIC RELATIONSHIPS AND BIOLOGICAL PROPERTIES OF SWINE INFLUENZA A/H1N1 VIRUS STRAINS ISOLATED IN NORTHERN KAZAKHSTAN IN 2018

N.T. Saktaganov, N.G. Klivleyeva, N.S. Ongarbayeva, T.I. Glebova, G.V. Lukmanova, A.M. Baimukhametova

Research and Production Center for Microbiology and Virology, A26T6C0 Republic of Kazakhstan, Almaty, st. Bogenbai Batyr, 105, e-mail biochem_vir@mail.ru, nsaktaganov1984@mail.ru (✉ corresponding author), i_nailya@list.ru, nuray.syrlybay@gmail.com, taty1962@mail.ru, gal_l@bk.ru, a_baimukhametova@mail.ru

ORCID:
Saktaganov N.T. orcid.org/0000-0001-6526-956X
Glebova T.I. orcid.org/0000-0002-1139-9868
Klivleyeva N.G. orcid.org/0000-0002-4094-137X
Lukmanova G.V. orcid.org/0000-0002-9809-6674
Ongarbayeva N.S. orcid.org/0000-0001-9022-331X
Baimukhametova A.M. orcid.org/0000-0002-8959-126X

Received October 21, 2019

 

Swine influenza is a highly contagious acute disease characterized by pronounced fever, general weakness, and disorders of the respiratory system. Swine influenza virus can cause disease in humans and, on the contrary, swine may be infected by human influenza virus. In the pig’s organism, simultaneously infected with viruses of different origin, genetic reassortment takes place with the risk of occurrence of new dangerous highly pathogenic strains. The study of influenza viruses circulating in the pig population therefore plays an important role in preventing the development of dangerous outbreaks of the disease and planning preventive measures. In this work we studied the characteristics of the newly isolated strains of swine influenza virus which are epizootically relevant in the specified region at the present time. Our purpose was to identify the biological and antigenic characteristics of strains of swine influenza A/H1N1virus, circulating in the North Kazakhstan oblast of the Republic of Kazakhstan in 2018. Influenza A/H1N1 virus strains were studied including those isolated from pigs in pig farms of the North Kazakhstan oblast, the A/swine/Petropavlovsk/01/18, A/swine/Petropavlovsk/02/18, and A/swine/Petropavlovsk/03/18, and also the reference strains A/swine/Iowa/15/30, A/swine/USA/1976/31, and A/California/04/09 pdm. The strains were cloned in 10-day-old developing chicken embryo systems. The antigenic properties of surface glycoproteins of the strains were examined by cross-reactivity hemagglutination inhibition assay with rabbit immune sera. Infectivity was determined in chicken embryos (CE) and MDCK cell culture. The adsorption properties were studied on formalinized chicken red blood cells under constant stirring at 4 °С for 18 hours. Elution from red blood cells was determined after 30, 60, 120, 180, and 240 min in buffered saline at 37 °С. The heat sensitivity of hemagglutinin was assessed by the ability to agglutinate red blood cells after heating at 56 °С for 5, 10, 15, 30, and 60 minutes. The hemagglutinating activity of strains was assayed using 0.75 % suspensions of chicken, guinea pig, ram, horse, and blood group I(0) individual  erythrocytes. The susceptibility of isolates to nonspecific inhibitors was determined in the hemagglutination inhibition assay with native and heated (30 min at 62 °С and 10 min at 100 °С) blood sera of guinea pig, chicken, and rabbit. The susceptibility of virus strains to different concentrations of antiviral drugs was evaluated by the level of reproductive suppression of lg 100 EID50/0.2 ml of virus in CE. The antigenic relationship of the examined variants of influenza A/H1N1 virus between each other and with the reference strains A/swine/USA/1976/31 and A/swine/Iowa/15/30 was revealed as well as their difference from the strain А/California/04/09 pdm. The studied strains in high titers agglutinated all types of red blood cells taken in the experiment. The infectious activity of swine influenza virus strains ranged within 6.5-7.9 lg EID50/0.2 ml in chicken embryos, and 3.5-4.3 lg TCD50/0.2 ml in MDCK cell culture. After heating at 56 °С, all strains agglutinated chicken erythrocytes in high titers (log2 = 6.3±0.6-9.6±0.8) and were characterized as thermostable. The isolated strains possessed good adsorption ability against chicken erythrocytes (90-100 %) and eluted from them after 30-60 min incubation at 37 °С. The strains revealed inhibitory resistance with native nonspecific sera and were suppressed by inhibitors of the heated sers only. The studied strains proved to be susceptible to Tamiflu and Remantadine (the inhibitory concentrations were 5.6-6.6 and 3.7-12.7 μg/ml, respectively). Virusesexhibited resistance to the drugs Arbidol and Ingavirin. Thus, the study revealed similarity of isolated and reference A/H1N1 strains in thermostability of the hemagglutinin, adsorption rate and susceptibility to antiviral drugs, as well as differences in infectious activity and the rate of elution from chicken red blood cells.

Keywords: swine influenza virus, A/H1N1, strain, isolate, antigen, hemagglutinin, infectivity, thermostability, resistance, drug susceptibility.

 

REFERENCES

  1. Lvov D.K. Influenza A viruses — a sum of populations with a common protected gene pool. In: Soviet medical reviews. Section E. Virology reviews. V.M. Zhdanov (ed.). Bell and Bain Ltd., Glasgow, 1987, V. 2: 15-37.
  2. Webster R.G., Bean W.J., Gorman O.T., Chambers T.M., Kawaoka Y.C. Evolution and ecology of influenza A viruses. Microbiological Reviews, 1992, 56(1): 152-179 CrossRef
  3. Hinshaw V.S., Webster R.G., Bean W.J., Downie J., Senne D.A. Swine influenza-like viruses in turkeys: potentional source of virus for humans? Science, 1983, 220(4593): 206-208 CrossRef
  4. Swine Influenza (Influenza in Swine). Available: https://www.cdc.gov/flu/swineflu/influenza-in-swine.htm. Accessed: 20.08.2019.
  5. VOZ. Vspyshka grippa sredi lyudei, vyzvannaya grippom A/H1N1, — soobrazheniya po povodu perekhoda virusa ot zhivotnogo k cheloveku. 30 aprelya 2009 goda [WHO. A/H1N1 influenza outbreak in humans — Considerations for the transition of the virus from animal to human. April 30, 2009]. Available: https://www.google.ru/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=
    rja&uact=8&ved=2ahUKEwi6sdz9s6niAhUNuIsKHep6AngQFjAAegQIARAC&url=
    http%3A%2F%2Forigin.who.int%2Fentity%2Ffoodsafety%2Ffs_management%
    2FNo_02_influenza_Apr09_ru_rev1.pdf&usg=AOvVaw1l43P4XoJ-JgAeEUsOOJDI. Accessed: 20.05.2019 (in Russ.).
  6. Simon G., Larsen L.E., Dürrwald R., Foni E., Harder T., Van Reeth K., Markowska-Daniel I., Reid S.M., Dan A., Maldonado J., Huovilainen A., Billinis C., Davidson I., Agüero M., Vila T., Hervé S., Breum S.Ø., Chiapponi C., Urbaniak K., Kyriakis C.S., ESNIP3 consortium, Brown I.H., Loeffen W. European surveillance network for influenza in pigs: surveillance programs, diagnostic tools and Swine influenza virus subtypes identified in 14 European countries from 2010 to 2013. PloS ONE, 2014, 9(12): e115815 CrossRef
  7. Lukmanova G.V., Klivleeva N.G., Shamenova M.G., Saktaganov N.T., Glebova T.I., Mustafin M.K., Baiseev G.A., Mustafin B.M. Veterinariya, 2016, 1(45): 61-63 (in Russ.).
  8. Klivleyeva N.G., Saktaganov N.T., Glebova T.I., Lukmanova M.G., Shamenova M.G., Sayatov M.H. Detection of influenza A(H1N1) viruses in humans and pigs in the Northern Kazakhstan during 2014-2016. Int. Conf. Symp. «Influenza 2018: Centenary of the 1918 Pandemic». London, 2018: 38.
  9. Saktaganov N.T., Klivleeva N.G., Glebova T.I., Kalkozhaeva M.K., Lukmanova G.V., Ongarbaeva N.S., Baiseiit S.B., Baimukhametova A.M., Shamenova M.G. Veterinariya, 2019, 2: 21-25 CrossRef (in Russ.).
  10. Brown I.N., Done S.N., Spencer Y.I., Cooley W.A., Harris P.A., Alexander D.J. Pathogenicity of a swine influenza H1N1 virus antigenically distinguishable from classical and European strains. Veterinary Record, 1993, 132(24): 598-602 CrossRef
  11. Factsheet on swine influenza in pigs. Available: https://www.ecdc.europa.eu/en/swine-influenza/facts/swine-influenza-pigs. Accessed: 20.05.2019.
  12. Ma J., Shen H., McDowell C., Liu Q., Duff M., Lee J., Lang Y., Hesse D., Richt J.A., Ma W. Virus survival and fitness when multiple genotypes and subtypes of influenza A viruses exist and circulate in swine. Virology, 2019, 532: 30-38 CrossRef
  13. Sominina A.A., Burtseva E.I., Lobova T.G., Konovalova N.I., Gudkova T.M., Litvinova O.M., Slepushkin A.N., Ivanova V.T. Vydelenie virusov grippa v kletochnykh kul'turakh i kurinykh embrionakh i ikh identifikatsii. Metodicheskie rekomendatsii (utverzhdeny FS po nadzoru v sfere zashchity prav potrebitelei i blagopoluchiya cheloveka). 18 aprelya 2006 g. № 0100/4430-06-34 [Isolation of influenza viruses in cell cultures and chicken embryos and their identification. Methodical recommendations (approved by the Federal Assembly for Supervision of Consumer Rights Protection and Human Well-being). April 18, 2006 No. 0100/4430-06-34]. Available: http://docs.cntd.ru/document/420214503. Accessed: 20.05.2019 (in Russ.).
  14. WHO Global Influenza Surveillance Network. Manual for the laboratory diagnosis and virological surveillance of influenza. WHO Press, Geneva, 2011. Available: http://apps.who.int/bo-okorders/MDIbookPDF/Book/11500806.pdf?ua=1. Accessed: 28.04.2018.
  15. Archetti I., Horsfall F.L. Jr. Persistent antigenic variation of influenza a viruses after incomplete neutralization in ovo with heterologous immune serum. Journal of Experimental Medicine, 1950, 92(5): 441-462 CrossRef
  16. Reed L., Muench H. A simple method of estimation fifty percent and pints. American Journal of Tropical Medicine and Hygiene, 1938, 27(20): 493-497.
  17. Cloud S.S., Rosenberger J.K. Characterization of nine avian paramyxoviruses. Avian Diseases, 1986, 24(1): 139-152 CrossRef
  18. Ito T., Suzuki Y., Mitnaul L., Vines A., Kida H., Kawaoka Y. Receptor specificity of influenza A viruses correlates with the agglutination of erythrocytes from different animal species. Virology, 1997, 227(2): 493-499 CrossRef
  19. Rukovodstvo po eksperimental'nomu (doklinicheskomu) izucheniyu novykh farmakologicheskikh veshchestv /Pod redaktsiei R.U. Khabrieva [Guidelines for the experimental (preclinical) study of new pharmacological substances. R.U. Khabriev (ed.)]. Moscow, 2005 (in Russ.).
  20. Tare D.S., Pawar S.D. Use of embryonated chicken egg as a model to study the susceptibility of avian influenza H9N2 viruses to oseltamivir carboxylate. Journal of Virological Methods, 2015, 224: 67-72 CrossRef
  21. Loginova S.Ya., Borisevich S.V., Maksimov V.A., Bondarev V.P. Antibiotiki i khimioterapiya, 2009, 54(3-4): 11-14 (in Russ.).
  22. Ongarbaeva N.S., Saktaganov N.T., Kalkozhaeva M.K., Lukmanova G.V., Baimukhametova A.M., Glebova T.I., Klivleeva N.G. Materialy IV Mezhdunarodnoi nauchnoi konferentsii «Dostizheniya molodykh uchenykh v veterinarnuyu praktiku», posvyashchennoi 55-letiyu aspirantury FGBU VNIIZZH [Proc. IV Int. Conf. «Achievements of young scientists in veterinary practice», dedicated to the 55th anniversary of postgraduate studies at FGBU VNIIZZh]. Vladimir, 2016: 99-103 (in Russ.).
  23. Kiselev O.I. Genom pandemicheskogo virusa grippa A/N1N1v-2009 [The genome of the pandemic influenza virus A/H1N1v-2009]. St. Petersburg-Moscow, 2011 (in Russ.).
  24. Tabynov K.K., Mamadaliev S.M., Azanbekova M.A., Mambetaliev M. Aktual'nye voprosy veterinarnoi biologii, 2009, 4(4): 8-11 (in Russ.).
  25. Cai M., Huang J., Bu D., Yu Z., Fu X., Ji C., Zhou P., Zhang G. Molecular evolution of H1N1 swine influenza in Guangdong, China, 2016-2017. Infection, Genetics and Evolution, 2018, 60: 103-108 CrossRef

 

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