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Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2019, Vol. 54, № 2, p. 304-315.
(how to cite this article)

doi: 10.15389/agrobiology.2019.2.304eng

UDC: 591.132:57.084.1:579.6

Acknowledgements:
Supported financially by the subprogram “Study of the mechanisms of adaptation of the digestive system of mammals and poultry to diets with different ingredient composition of feed” (Decree of the Presidium of the Russian Academy of Sciences No. 132 of 05.07.2017) within the framework of the state task 0761-2018-0031

 

TDIFFERENT CHROME SOURCES INFLUENCE ON MORPHO-BIOCHEMICAL INDICATORS AND ACTIVITY OF DIGESTIVE ENZYMES IN WISTAR RATS

S.V. Lebedev1, 2, I.A. Gavrish 1, 2, I.Z. Gubaydullina1

1Federal Research Centre of Biological Systems and Agrotechnologies RAS, 29, ul. 9 Yanvarya, Orenburg, 460000 Russia, e-mail lsv74@list.ru (✉ corresponding author), gavrish.irina.ogu@gmail.com, gubaidullinae@mail.ru;
2Orenburg State University, 13, prosp. Pobedy, Orenburg, 460018 Russia

ORCID:
Lebedev S.V. orcid.org/0000-0001-9485-7010
Gubaydullina I.Z. orcid.org/0000-0001-7862-3660
Gavrish I.A. orcid.org/0000-0002-9377-7673

Received November 7, 2018

 

Nowadays, issues of mineral nutrition of humans and animals are quite relevant. High-energy rations, multicomponent feed mixtures and additives in the diets require special attention when optimizing limited microelements. A priori, chromium, being an important trace element in animals, is used to correct carbohydrate, fat and lipid metabolism. Due to its low content in the components of diets, its role in the formation of the microecological status of the body is poorly understood. At the same time, its biological availability in the body depends on the source of chromium. In the present work, using a model object, the Wistar rats, we for the first time compared the biological effects of various chromium sources, i.e. picolinate (CrPic), nanoform (NP Cr2О3) and chloride (CrCl3) at doses of 300 and 500 μg/kg feed, according to a set of indicators (feed digestibility, hematological parameters, activity of digestive enzymes, composition of intestinal microflora) and established greater bioavailability and more pronounced positive effect of picolinate and chromium nanoparticles on body weight and hematological parameters and ambiguous influence of the studied forms on the activity of digestive enzymes and intestinal microflora. The purpose of this work was to study the biological effect of chromium in various forms and dosages on Wistar rats. The studies were carried out on 105 white male rats weighing 70-80 g under standard vivarium conditions (Federal Scientific Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences). Animals were divided into seven groups (n = 15 each). The control group was fed a common diet. The group I diet included Cr2О3 NPs at a dose of 300 µg/kg feed (NP 300), group II — CrCl3 at a dose of 300 mg/kg (CrCl3 300), group III — chromium picolinate (CrPic) at a dose of 300 mg/kg (CrPic 300), group IV — Cr2О3 NPs at a dose of 500 µg/kg of feed (NP 500), group V  — CrCl3 at a dose of 500 mg/kg (CrCl3 500), and group VI — CrPic at a dose of 500 mg/kg (CrPic 500). Nanoparticles were introduced into the feed by mixing. The introduction of chromium in the form of CrPic and Cr2О3 NPs at a dose of 500 μg/kg, under the same feed consumption, was accompanied by an increase in the body weight of rats by 22.6 and 22.2 % (p ≤ 0.05). The effect of Cr2О3 NPs expressed as the absence of the reaction of lymphocytes, monocytes and granulocytes, in other cases their level exceeded control values by 14 to 45 %. Synthesis of hemoglobin was adequate to stimulation of erythropoiesis, but an increase in the number of platelets resulted in blood sludging, an increase in viscosity and difficulty in perfusion through vessels. This symptom was typical for NP Cr2О3 300, CrCl3 300 and CrCl3 500 (the difference with the control is from 70 to 90 %, p ≤ 0.05). High digestibility of CrPic and NPs Cr2О3 500 (from 20.2 to 34.0 %), accompanied by manifestation of hepato- and nephrotoxicity, with signs of oxidative stress, decreased activity of amylase and lipase in the blood plasma, indicating a depressant effect of high doses of chromium on enteropancreatic circulation of the digestive enzymes and metabolic disorders of Mg and Fe in the blood. Triglycerides, like true fats, decreased at maximum doses of chromium in the form of chloride and picolinate, confirming their participation in lipid metabolism, causing splitting of excess fat in the body, and reducing the ratio of fat to body weight ratio from 2 (control group) to 0.82 (NP Cr2О3 500). The indicators of bilirubin and creatinine clearly demonstrate the absence of toxicity at low doses, i.e. Cr2О3 NPs 300 and CrPic 300. Amylase activity in the pancreas is increased at a dose of 300 µg/kg of Cr2О3 NPs. Dietary CrPic in a similar dosage stimulated the activity of lipase and protease, whereas in the 12 duodenal ulcer it led to a decrease in the activity of amylase and lipase. CrCl3 and CrPic at a dosage of 500 µg/kg reduced the activity of lipase in the duodenum. The specific effect of Cr NPs 500 µg/kg on the microecological status of the organism was manifested in a decrease in the number of lactic acid bacteria by 55.9 %. The number of bifidobacteria was significantly higher, by 48.6 % (p £ 0.05), in the PicCr 500-fed group. The number of enterobacteria in the NP 300-fed group was 34.8 % lower than the control, while in the other groups their number increased 24.0-33.7 times (р ≤ 0.05). From the totality of the estimated parameters of the intestinal microflora, the use of nanoparticles in the composition of the diet is promising due to the lack of resistance. Thus, chromium of CrPic 500, NP Cr2О3 300 does not show a toxic effect on the body, and has a stimulating effect on growth, development, digestibility of chromium, the activity of the digestive enzymes and microecological status of the organism, which puts these forms in the category of promising sources of chromium for the correction of metabolism and the microbial composition of the gastrointestinal tract of animals.

Keywords: rats, chromium concentration, productivity, blood biochemical parameters, digestive enzymes, intestinal microflora.

 

 

REFERENCES

  1. Campbell W.J., Mertz W. Interaction of insulin and chromium (III) on mitochondrial swelling. American Journal of Physiology-Legacy Content, 1963, 204(6): 1028-1030 CrossRef
  2. Mertz W., Roginski E.E. Chromium metabolism: the glucose tolerance factors. American Journal of Clinical Nutrition, 1971, 33: 163-239.
  3. Hasan H.G., Mahmood T.J., Ismael P.A. Studies on the relationship between chromium (III) ion and thyroid peroxidase activity in sera of patients with thyroid dysfunction. Ibn AL-Haitham Journal for Pure and Applied Science, 2017, 24(2): 1-10.
  4. Shukla A., Shukla J.P. Hexavalent chromium induced alterations in the nucleic acids and protein metabolism in the liver of the fingerlings of freshwater siluroid, Mystus (M.) vittatus (Bl.) Int. J. Pharm. Sci. Res., 2016, 10: 20 CrossRef
  5. Okada S., Tsukada H., Tezuka M. Effect of chromium (III) on nucleolar RNA synthesis. Biological Trace Element Research, 1989, 21(1): 35-39 CrossRef
  6. Vincent J.B. Chromium: basic nutritional and toxicological aspects. In: Molecular, genetic, and nutritional aspects of major and trace minerals. J. Collins (ed.). Academic Press, 2016.
  7. Vincent J. The nutritional biochemistry of chromium (III). Elsevier, 2018.
  8. Sahin K., Sahin N., Onderci M., Gursu F., Cikim G. Optimal dietary concentration of chromium for alleviating the effect of heat stress on growth, carcass qualities, and some serum metabolites of broiler chickens. Biological Trace Element Research, 2002, 89(1): 53-64 CrossRef
  9. Onderci M., Sahin N., Sahin K., Kilic N. Antioxidant properties of chromium and zinc. Biological Trace Element Research, 2003, 92(2): 139-149 CrossRef
  10. Kornegay E.T., Wang Z., Wood C.M., Lindemann M.D. Supplemental chromium picolinate influences nitrogen balance, dry matter digestibility, and carcass traits in growing-finishing pigs. Journal of Animal Science, 1997, 75(5): 1319-1323 CrossRef
  11. Florence A.T. The oral absorption of micro-and nanoparticulates: neither exceptional nor unusual. Pharmaceutical Research, 1997, 14(3): 259-266 CrossRef
  12. Desai C., Jain K., Madamwar D. Evaluation of in vitro Cr (VI) reduction potential in cytosolic extracts of three indigenous Bacillus sp. isolated from Cr (VI) polluted industrial landfill. Bioresource technology, 2008, 99(14): 6059-6069 CrossRef
  13. National Research Council (NRC) Mineral tolerance of animals. Second revised edition, Committee on minerals and toxic substances in diets and water for animals, Board on agriculture and natural resources, Division on earth and life studies. National Academy Press, Washington D.C, 2005.
  14. Lien T.F., Yeh H.S., Lu F.Y., Fu C.M. Nanoparticles of chromium picolinate enhance chromium digestibility and absorption. Journal of the Science of Food and Agriculture, 2009, 89(7): 1164-1167 CrossRef
  15. Wang M.Q., Xu Z.R. Effect of chromium nanoparticle on growth performance, carcass characteristics, pork quality and tissue chromium in finishing pigs. Asian-Australasian Journal of Animal Sciences, 2004, 17(8): 1118-1122 CrossRef 
  16. Wang M.Q., Xu Z.R., Zha L.Y., Lindemann M.D. Effects of chromium nanocomposite supplementation on blood metabolites, endocrine parameters and immune traits in finishing pigs. Animal Feed Science and Technology, 2007, 139(1-2): 69-80 CrossRef
  17. Zha L.Y., Wang M.Q., Xu Z.R., Gu L.Y. Efficacy of chromium(III) supplementation on growth, body composition, serum parameters, and tissue chromium in rats. Biological Trace Element Research, 2007, 119(1): 42-50 CrossRef
  18. Coles E.H. Veterinary clinical pathology. W.B. Saunders Company, Philadelphia, 1986.
  19. Boutwell J.H. Clinical chemistry. Laboratory manual and methods. Journal of Medical Education, 1962, 37(2): 158.
  20. Batoev Ts.Zh. V sbornike: Trudy Buryatskogo SKHI [In: Proceedings of the Buryat Agricultural Institute]. Ulan-Ude, 1971, vyp. 25: 122-126 (in Russ.).
  21. Gaziumarova L.D., Titov L.P., Klyuiko N.L. Bakteriologicheskaya diagnostika disbakterioza kishechnika: instruktsiya po primeneniyu [Bacteriological diagnosis of intestinal dysbiosis: instructions]. Leningrad-Minsk, 2010 (in Russ.).
  22. Oberlis D., Kharland B., Skal'nyi A. Biologicheskaya rol' makro- i mikroelementov u cheloveka i zhivotnykh [Biological role of macro- and microelements in humans and animals]. St. Petersburg, 2008 (in Russ.). 
  23. Hussain N., Jaitley V., Florence A.T. Recent advances in the understanding of uptake of microparticulates across the gastrointestinal lymphatics. Advanced Drug Delivery Reviews, 2001, 1(50): 107-142 CrossRef
  24. Sizova E., Yausheva E., Miroshnikov S., Lebedev S., Duskaev G. Element status in rats at intramuscular injection of iron nanoparticles. OSPC — Biosciences, Biotechnology Research Asia, 2015, 12(2): 119-127.
  25. Underwood E.J. Chromium. In: Trace elements in human and animal nutrition /E.J. Underwood (ed.). Academic Press, New York, 1997.
  26. Oberleas D., Kwun I.S. Confirmation of the mechanism of zinc homeostasis. In: Metal ions in biology and medicine-international symposium, vol. 5. P. Collery, P. Bratter, V. Negretti de Bratter, L. Khassanova, J.C. Etienne (eds.). John Libbey Eurotext, Paris, 1998: 140-145.
  27. Sizova E.A., Miroshnikov S.A., Kalashnikov V.V. Morphological and biochemical parameters in Wistar rats influenced by molybdenum and its oxide nanoparticles. Sel’skokhozyaistvennaya Biologiya [Agricultural Biology], 2016, 51(6): 926-936 CrossRef 
  28. Yausheva E.V., Miroshnikov S.A., Kvan O.V. Vestnik Orenburgskogo gosudarstvennogo universiteta, 2013, 12(161): 203-207 (in Russ.). 
  29. Bagchi D., Sidney J, Downs B.W., Bagchi M., Preuss H.G. Cytotoxicity and oxidative mechanisms of different forms of chromium. Toxicology, 2002, 180(1): 5-22 CrossRef
  30. Sargeant T., Lim T.H., Jenson R.L. Reduced chromium retention in patients with hemochromatosis: a possible basis of hemochromatotic diabetes. Metabolism, 1979, 28(1): 70-79 CrossRef
  31. Ani M., Moshtaghie A.A. The effect of chromium on parameters related to iron metabolism. Biological Trace Element Research, 1992, 32(1-3): 57-64 CrossRef
  32. Shamsutdinova I.R., Derkho M.A.. Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta, 2015, 6(56): 122-124 (in Russ.). 
  33. Speetjens J.K., Collins R.A., Vincent J.B., Woski S.A. The nutritional supplement chromium(III) tris(picolinate) cleaves DNA. Chemical Research in Toxicology, 1999, 12(6): 483-487 CrossRef
  34. Mooney K.W., Cromwell G.L. Efficacy of chromium picolinate and chromium chloride as potential carcass modifiers in swine. Journal of Animal Science, 1997, 75(10): 2661-2671 CrossRef
  35. Karajanagi S.S., Vertegel A.A., Kane R.S., Dordick J.S. Structure and function of enzymes adsorbed onto single-walled carbon nanotubes. Langmuir, 2004, 20(26): 11594-11599 CrossRef
  36. Vertegel A.A., Siegel R.W., Dordick J.S. Silica nanoparticle size influences the structure and enzymatic activity of adsorbed lysozyme. Langmuir, 2004, 20(16): 6800-6807 (doi:  10.1021/la0497200).
  37. Win K.Y., Feng S.S. Effects of particle size and surface coating on cellular uptake of polymeric nanoparticles for oral delivery of anticancer drugs. Biomaterials, 2005, 26(15): 2713-2722 CrossRef

 

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